Journal of Postgraduate Medicine
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Year : 1994  |  Volume : 40  |  Issue : 1  |  Page : 7-9

Determination of pyrazinamide in human by high performance liquid chromatography.


Medical Department, Hindustan Ciba Geigy Research Centre, Bombay, Maharashtra.

Correspondence Address:
S N Revankar
Medical Department, Hindustan Ciba Geigy Research Centre, Bombay, Maharashtra.

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Source of Support: None, Conflict of Interest: None


PMID: 0008568720

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A facile and sensitive high performance liquid chromatographic (HPLC) technique has been developed for the determination pyrazinamide (PZA) in human plasma. Nicotinamide(NIA) is used as internal standard(IS). Plasma is deproteinized with 0.7 M perchloric acid; clear supernatant is neutralized with 1M NaOH and injected onto HPLC. The separation of pyrazinamide and the internal standard is carried out on a Supelco LC-18 (DB) column with a basic mobile phase. Pyrazinoic acid, the major metabolite, other anti-tuberculous drugs and endogenous components do not interfere with measurement of pyrazinamide. The limit of detection of pyrazinamide with this method is 0.2 mg/0.2 ml plasma (CV 8.2%).






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Online since 12th February '04
2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow