Journal of Postgraduate Medicine
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Year : 1996  |  Volume : 42  |  Issue : 3  |  Page : 72-5

Viability testing of homograft valves using methyl thiazol tetrazolium assay.


Department of Microbiology, Lokmanya Tilak Municipal Medical College, Sion, Mumbai.

Correspondence Address:
S S Shetty
Department of Microbiology, Lokmanya Tilak Municipal Medical College, Sion, Mumbai.

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Source of Support: None, Conflict of Interest: None


PMID: 0009715320

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Viability status before and after treatment with antibiotics was investigated in a total of 104 homograft valves using MTT (3-[4, 5-dimethyl thiazol-2-y1]-2, 5-diphenyl tetrazolium bromide). The valves with warm ischaemic time above 11 hours, were found to be non-viable. Increase in storage time directly decreases cell viability. Methyl thiazol tetrazolium (MTT) assay can be used as a reliable, simple, rapid and economic method for assessing the viability status based on mitochondrial respiration even for homograft valves. Basal media with and without nutrients i.e., DMEM and Hanks BSS showed no difference in viability of the cells.






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Online since 12th February '04
2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow