Journal of Postgraduate Medicine
 Open access journal indexed with Index Medicus & ISI's SCI  
Users online: 4151  
Home | Subscribe | Feedback | Login 
About Latest Articles Back-Issues Article Submission Resources Sections Etcetera Contact
 
  NAVIGATE Here 
  Search
 
 :: Next article
 :: Previous article 
 :: Table of Contents
  
 RESOURCE Links
 ::  Similar in PUBMED
 ::  Search Pubmed for
 ::  Search in Google Scholar for
 ::Related articles
 ::  Article in PDF (254 KB)
 ::  Citation Manager
 ::  Access Statistics
 ::  Reader Comments
 ::  Email Alert *
 ::  Add to My List *
* Registration required (free) 

  IN THIS Article
 ::  Abstract
 ::  Patients and methods
 ::  Results
 ::  Discussion
 ::  References
 ::  Article Tables

 Article Access Statistics
    Viewed9667    
    Printed190    
    Emailed5    
    PDF Downloaded187    
    Comments [Add]    
    Cited by others 8    

Recommend this journal


 


 
BRIEF REPORT
Year : 2002  |  Volume : 48  |  Issue : 4  |  Page : 266-9

The role of ACE gene polymorphism in rapidity of progression of focal segmental glomerulosclerosis.


Department of Paediatrics, Steele Memorial Children's Research Centre, University of Arizona, Tucson AZ 85724, USA. , USA

Correspondence Address:
M Dixit
Department of Paediatrics, Steele Memorial Children's Research Centre, University of Arizona, Tucson AZ 85724, USA.
USA
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 12571380

Rights and PermissionsRights and Permissions


 :: Abstract 

BACKGROUND: The insertion/deletion (I/D) polymorphism of angiotensin converting enzyme (ACE) gene has been associated with progression of renal diseases. AIMS: We investigated its role in the rate of progression of focal segmental glomerulosclerosis (FSGS). METHODS: Forty-seven patients with end-stage renal disease (ESRD) due to FSGS were evaluated. RESULTS: The distribution of ACE genotype was II-25.5%, ID-55.5%, and DD-19%, as compared to 40 controls with genotype of 7.5%, 60%, and 32.5%, respectively (p= NS). In African Americans (AA) the gene frequencies among patients and controls were I-43%, D-57% vs I-36%, D-64%, respectively. This was different than the gene frequencies in White/Hispanic (W/H) patients I-61.5%, D-38.5% vs I-38.6%, D-61.4%, in controls (P < 0.05). In 22 patients with rapid progression (RP) of FSGS to ESRD the genotype distribution was II-18%, ID -64%, and DD-18%. In 25 patients with FSGS who progressed slowly (SP) the genotype was similar (II-32%, ID-48% and DD-20%, P >0.05). With respect to rate of progression, D allele frequency was similar in AA patients (RP 64% vs SP 50%) and W/H patients (RP 36% vs SP 40%). CONCLUSION: Our study reveals no association between the I/D polymorphism of the ACE gene and the presence of and rapidity progression of FSGS.


Keywords: Adolescent, Adult, Alleles, Caucasoid Race, genetics,Child, Disease Progression, Female, Genotype, Glomerulosclerosis, Focal, genetics,Human, Male, Middle Age, Negroid Race, genetics,Peptidyl-Dipeptidase A, genetics,Polymorphism (Genetics),


How to cite this article:
Dixit M, Mansur A, Dixit N, Gilman J, Santarina L, Glicklich D. The role of ACE gene polymorphism in rapidity of progression of focal segmental glomerulosclerosis. J Postgrad Med 2002;48:266

How to cite this URL:
Dixit M, Mansur A, Dixit N, Gilman J, Santarina L, Glicklich D. The role of ACE gene polymorphism in rapidity of progression of focal segmental glomerulosclerosis. J Postgrad Med [serial online] 2002 [cited 2019 Nov 12];48:266. Available from: http://www.jpgmonline.com/text.asp?2002/48/4/266/82


Idiopathic focal segmental glomerulosclerosis (FSGS) is one of the most important diseases leading to progressive renal failure in adults and children. A number of observations suggest that genetic factors may play an important role in progression of FSGS.[1],[2] A polymorphism of the angiotensin converting enzyme (ACE) gene consisting of a 287 bp fragment within intron 16 defined by insertion (I) or deletion (D) was reported to be associated with progression of several renal diseases including IgA nephropathy, autosomal dominant polycystic kidney disease and diabetic nephropathy.[3],[4],[5] In a Korean population the double deletion (DD) genotype in FSGS patients has been associated with steroid unresponsiveness and a tendency to progress to renal failure.[6] A strong genetic association has also been found between a DD genotype and increased risk of atherosclerotic cardio-vascular and cerebral diseases.[7],[8] In African Americans (AA) end-stage renal disease (ESRD) due to FSGS occurs much more frequently than in the general population.[9] AA with essential hypertension have a higher frequency of DD genotype than normotensive AA.[10] The rate of progression of FSGS varies among different races. FSGS can be particularly aggressive in AA with the rate of progression to ESRD from the time of diagnosis is significantly less than other racial groups.[11],[12],[13]

The purpose of the present study was to determine if I/D polymorphism of intron 16 of the ACE gene is associated with FSGS and with its high rate of progression, particularly in African Americans.


  ::   Patients and methods Top


Seventy ESRD patients with biopsy proven FSGS were identified. Twenty-three patients were excluded from the study because of a systemic disease being the most likely etiology of FSGS. The patients were recruited from the dialysis unit or from the Nephrology clinic at Albert Einstein College of Medicine and allied hospitals. Of the 47 patients with primary FSGS included in the study, 21 patients were African-Americans (AA) and 26 were White/Hispanics (W/H). Forty healthy blood donors (18 AA and 22 W/H) served as controls. The controls had normal blood pressure and no history of renal disease at the time of blood donation. Patient charts were reviewed with special emphasis on race, time of biopsy and time of ESRD. The disease was called rapidly progressive (RP) when the time period between initial biopsy and ESRD exceeded two years. The disease was considered to be slowly progressive (SP) when the period from the diagnostic renal biopsy to ESRD was longer than two years. This study was approved by the Institutional Review Board at Albert Einstein College of Medicine and allied hospitals. Written informed consent was obtained from the patient/guardian and the donor in each case. The study was completed within 12 months from the time of initiation.

Ten ml blood was collected from patients and controls and stored at -200C pending analysis. DNA was isolated from peripheral leucocytes by the standard protocol. The I/D polymorphism in intron 16 was determined by a PCR based protocol.[4] As there may be preferential amplification of the D allele,[4] all samples found to have DD after amplification with conventional primers, were re-amplified with an insertion-specific primer pair.[4],[5] The distribution of genotypes and alleles were compared among patients and controls using ?2 tests.


  ::   Results Top


[Table - 1] lists selected characteristics of the patient and control groups. The time to ESRD from diagnosis of FSGS in AA patients was 2.9 ? 1.9 years as compared to 5.7 ? 4.5 years in W/H group; (p < 0.05).

Majority of patients (41/47) had nephrotic range proteinuria at the time of the kidney biopsy. The age at the time of the study, the male-female ratio, age at presentation, and incidence of nephrotic syndrome were not significantly different between AA and W/H patient groups.

The distribution of ACE genotype in 47 patients with FSGS was as follows: II 25.5%, ID 55.5%, and DD 19%, as compared to 40 controls, in whom the genotype was 7.5%, 60%, and 32.5%, respectively (P >0.05) [Table - 2]. The ACE genotype amongst the AA and W/H groups was similar. The gene frequencies in the AA group were similar between patients and controls (I 43%, D 57% vs I 36%, D 64%, respectively). However, there were significant differences in gene frequencies between the two groups of W/H individuals I 61.5%, D 38.5% vs I 38.6%, D 61.4%, in controls (P < 0.05).

Eleven AA and eleven W/H patients progressed rapidly (RP) [Table - 3]. In the RP group the genotype distribution was II 18%, ID 64%, and DD 18%. Among the 25 patients who progressed slowly (SP) the genotype was II 32%, ID 48% and DD 20% (P >0.05). But in the RP group 4/11 AA had a DD genotype as compared to 0/11 in W/H patients (P >0.05). With respect to the rate of progression, D allele frequency was similar between AA (RP 64% vs SP 50%, P >0.05) and W/H (RP 36% vs SP 40%, P >0.05).


  ::   Discussion Top


This study was undertaken to determine whether I/D polymorphism of intron 16 of the ACE gene is associated with ESRD due to FSGS. The result failed to support such an association. This is in agreement with study conducted by Burg et al who were unable to detect an association of the ACE gentotype with primary glomerulonephritis including 17 patients with FSGS.[14] In contrast, Lee reported that DD genotype in a Korean sample population was associated with poor response to steroids and tendency to progression.[6] This may be the result of genetic differences between the populations or due to relatively small numbers of patients studied. The DD genotype has been associated with accelerated progression in a variety of renal diseases such as IgA nephropathy,[4],[5] and polycystic kidney disease.[3] Marre and co-workers reported that the I/D polymorphism is associated with development of diabetic nephropathy,[15] whereas others did not confirm this relationship.[16] AA patients with essential hypertension have a significantly higher frequency of a DD genotype as compared to normotensive AA individuals.[17] However, the linkage analysis between loci in the renin-angiotensin axis and ESRD in AA population failed to suggest an association.[18] If the linkage between the disease locus and the I/D polymorphism varies among populations, positive association will be found in populations with tighter linkage but not in populations with weaker linkage. The frequency of DD genotype among dialysis patients is not increased as compared to general population.[19]

Renal survival is worst in FSGS with nephrotic syndrome (NS).[11],[13] 41/47 of our patients had NS. Genetic background also influences prognosis. FSGS tends to progress more rapidly in AA patients especially in the New York area.[11],[12],[13] Ingulli described 38 AA children in whom the time of progression to ESRD from the time of biopsy was 2.8 ? 1.3 year.[11] Detwiler described 14 patients, five of whom were on dialysis and three of whom had died of complications of renal failure within 15 months of diagnosis.[12] Valeri et al described 43 predominantly AA patients with idiopathic collapsing FSGS, in whom median time from diagnosis to ESRD was 13 months.[13] Though, we did not attempt to analyze our data on the basis of biopsy appearances in our study, the rate of progression to ESRD in AA patients was 2.9 ? 1.9 yrs as compared to 5.7 ? 4.5 yrs in W/H patients (p< 0.05). Based on the above information defining the RP patients as those who reached ESRD in less than two years from the time of biopsy, though somewhat arbitrary, seems justified. We did not find differences in the ACE genotype between the RP and SP groups. In fact, when we compared the RP group to patients who took longer than five years to reach ESRD there was no difference in genotype (data not shown). Yet, the higher frequency of the DD genotype among the AA RP than the W/H patients (4/11 vs 0/11) did not reach statistical significance probably because of the small number of patients in each group. To reconcile these differences, multi-center studies including larger numbers of patients are currently being conducted.

The exact mechanism by which the presence of the DD genotype leads to renal disease operates at the cellular level.[4] Caucasians with DD genotype have serum ACE levels and intra-cellular ACE activity twice those of II genotype.[20],[21] Higher ACE activity leads to increased Angiotensin II (AII) levels, which in turn promotes proliferation of mesangial cells and matrix.[22] AII promotes expression of growth factors which in turn leads to glomerulosclerosis.[23] In experimental models of chronic renal disease[24] and in human diabetic nephropathy[25] pharmacological blockade of ACE significantly slows down the rate of decline in renal function. The data regarding the relationship between response to ACE inhibition and DD genotype has been conflicting. A good correlation was found in IgA nephropathy and diabetic nephropathy,[4],[26] but others did not confirm this finding in primary glomerulonephritis[14] and in patients with proteinuria.[27] Studies in children have confirmed similar findings in different ethnic backgrounds.[28] In our study the overall frequency of 38.6% for the I allele and 61.4% for the D allele among W/H controls is similar to that found among Caucasians.[29] Therefore the significant difference in I and D allele frequency (61.5 and 38.5%, respectively, p< 0.05) between W/H with FSGS and W/H controls is noteworthy. There are two limitations to our study. First, it is a single centre experience that reflects a certain referral population. Secondly, due to small numbers in each group a type II error in statistical evaluation cannot be fully excluded. To the best of our knowledge this is the first study of its kind comparing DD genotype with rapidity of progression of FSGS resulting in ESRD, especially in an AA population.

In conclusion, our data reveals no association between the D allele of the ACE gene and the presence of or rapidity of progression of FSGS in AA and W/H patients. A multi-center study with a large number of patients is needed to further study the issue.

 
 :: References Top

1.Glicklich D, Haskell L, Sentizer D, Weiss RA. Possible genetic predisposition to idiopathic focal segmental glomerulosclerosis. Am J Kid Dis 1988;12:26-30.  Back to cited text no. 1    
2.Weening JJ, Beukers JJ, Grond J, Elema JD. Genetic factors in focal segmental glomerulosclerosis. Kidney Int 1986;29:789-98.  Back to cited text no. 2    
3.Baboolal K, Ravine D, Daniels J, Williams N, Holmans P, Coles GA, et al. Association of the angiotensin I converting enzyme gene deletion polymorphism with early onset of ESRF in PKD1 adult polycystic kidney disease. Kidney Int 1997;52:607-13.  Back to cited text no. 3    
4.Hunley TE, Julian BA, Phillips JA 3rd, Summar ML, Yoshida H, Horn RG, et al. Angiotensin converting enzyme gene polymorphism: potential silencer motif and impact on progression in IgA nephropathy. Kidney Int 1996;49:571-7.  Back to cited text no. 4    
5.Yoshida H, Kon V, Ichikawa I. Polymorphisms of the renin-angiotensin system genes in progressive renal disease. Kidney Int 1996;50:732-44.  Back to cited text no. 5    
6.Lee DY, Kim W, Kang SK, Koh GY, Park SK. Angiotensin converting enzyme gene polymorphism in patients with minimal-change nephrotic syndrome and focal segmental glomerulosclerosis. Nephron 1997;77:471-3.  Back to cited text no. 6    
7.Cambien F, Poirier O, Lecerf L, Evans A, Cambou JP, Arveiler D, et al. Deletion polymorphism in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction. Nature 1992;359: 641-4.  Back to cited text no. 7    
8.Doi Y, Yoshinari M, Yoshizumi H, Ibayashi S, Wakisaka M, Fujishima M. Polymorphism of the angiotensin-converting enzyme (ACE) gene in patients with thrombotic brain infarction. Atherosclerosis 1997;132: 145-50.  Back to cited text no. 8    
9.United States Renal Data System. USRDS 2000 Annual Data report. Bethedsa, MD The National Institute of Diabetes and Digestive and Kidney Diseases.  Back to cited text no. 9    
10.Rutledge DR, Kubilis P, Browe CS, Ross EA. Polymorphism of the angiotensin I converting enzyme gene in essential hypertensive patients. Biochem Mol Biol Int 1995;35:661-8.  Back to cited text no. 10    
11.Ingulli E, Tejani A. Racial differences in the incidence and renal outcome of idiopathic focal segmental glomerulosclerosis in children. Pediatr Nephrol 1991;5:393-7.  Back to cited text no. 11    
12.Detwiler RK, Falk RJ, Hogan SL, Jennette JC Collapsing glomerulopathy: a clinically and pathologically distinct variant of focal segmental glomerulosclerosis. Kidney Int 1994;45:1416-24.  Back to cited text no. 12    
13.Valeri A, Barisoni L, Appel GB, Seigle R, D’Agati V. Idiopathic collapsing focal segmental glomerulosclerosis: a clinicopathologic study. Kidney Int 1996;50:1734-46.  Back to cited text no. 13    
14.Burg M, Menne J, Ostendorf T, Kliem V, Floege J. Gene-polymorphisms of angiotensin converting enzyme and endothelial nitric oxide synthase in patients with primary glomerulonephritis. Clin Nephrol 1997;48:205-11.  Back to cited text no. 14    
15.Marre M, Jeunemaitre X, Gallois Y, Rodier M, Chatellier G, Sert C, et al. Contribution of genetic polymorphism in the renin-angiotensin system to the development of renal complications in insulin-dependent diabetes: Genetique de la Nephropathie Diabetique (GENEDIAB) study group. J Clin Invest 1997;99:1585-95.  Back to cited text no. 15    
16.Freire MB, van Dijk DJ, Erman A, Boner G, Warram JH, Krolewski AS. DNA polymorphisms in the ACE gene, serum ACE activity and the risk of nephropathy in insulin-dependent diabetes mellitus. Nephrol Dial Transplant 1998;13:2553-8.  Back to cited text no. 16    
17.Rutledge DR, Kubilis P, Browe CS, Ross EA. Polymorphism of the angiotensin I converting enzyme gene in essential hypertensive patients. Biochem Mol Biol Int 1995;35:661-8.  Back to cited text no. 17    
18.Yu H, Bowden DW, Spray BJ, Rich SS, Freedman BI. Linkage analysis between loci in the renin-angiotensin axis and end-stage renal disease in African Americans. J Am Soc Nephrol 1996;7:2559-64.  Back to cited text no. 18    
19.Nomura H, Koni I, Michishita Y, Morise T, Takeda R. Angiotensin-converting enzyme gene polymorphism in haemodialysis patients. Lancet 1994;343:482-3  Back to cited text no. 19    
20.Costerousse O, Allegrini J, Lopez M, Alhenc-Gelas F. Angiotensin I-converting enzyme in human circulating mononuclear cells: genetic polymorphism of expression in T-lymphocytes. Biochem J 1993;290: 33-40.  Back to cited text no. 20    
21.Rigat B, Hubert C, Alhenc-Gelas F, Cambien F, Corvol P, Soubrier F. An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest 1990;86:1343-6.  Back to cited text no. 21    
22.Ray PE, Bruggeman LA, Horikoshi S, Aguilera G, Klotman PE. Angiotensin II stimulates human fetal mesangial cell proliferation and fibronectin biosynthesis by binding to AT1 receptors. Kidney Int 1994;45: 177-84.  Back to cited text no. 22    
23.Ruiz-Ortega M, Gomez-Garre D, Alcazar R, Palacios I, Bustos C, Gonzalez S, et al. Involvement of angiotensin II and endothelin in matrix protein production and renal sclerosis. J Hypertens 1994;12(Suppl):4S51-S58.  Back to cited text no. 23    
24.Kakinuma Y, Kawamura T, Bills T, Yoshioka T, Ichikawa I, Fogo A. Blood pressure-independent effect of angiotensin inhibition on vascular lesions of chronic renal failure. Kid Int 1992;42:46-55.  Back to cited text no. 24    
25.Lewis EJ, Hunsicker LG, Bain RP, Rohde RD. The effect of angiotensin-converting-enzyme inhibition on diabetic nephropathy. The Collaborative Study Group. N Engl J Med 1993;329:1456-62.  Back to cited text no. 25    
26.Mitch WE Is the inherited ACE genotype a trump or a joker? J Clin Invest 1995;96:2100-1.  Back to cited text no. 26    
27.van der Kleij FG, Navis GJ, Gansevoort RT, Heeg JE, Scheffer H, de Zeeuw D, et al. ACE polymorphism does not determine short-term renal response to ACE-inhibition in proteinuric patients. Nephrol Dial Transplant 1997;12(Suppl 2):42-6.  Back to cited text no. 27    
28.Frishberg Y, Becker-Cohen R, Halle D, Feigin E, Eisenstein B, Halevy R, et al. Genetic polymorphisms of the renin-angiotensin system and the outcome of focal segmental glomerulosclerosis in children. Kidney Int 1998;54:1843-9.  Back to cited text no. 28    
29.Morris BJ, Zee RY, Schrader AP. Different frequencies of angiotensin-converting enzyme genotypes in older hypertensive individuals. J Clin Invest 1994;94:1085-9.  Back to cited text no. 29    


    Tables

[Table - 1], [Table - 2], [Table - 3]

This article has been cited by
1 Analysis of polymorphism in Renin Angiotensin System and other related genes in South Indian chronic kidney disease patients
Anbazhagan, K., Sampathkumar, K., Ramakrishnan, M., Gomathi, P., Gomathi, S., Selvam, G.S.
Clinica Chimica Acta. 2009; 406((1-2)): 108-112
[Pubmed]
2 Angiotensin-converting enzyme gene polymorphism and the progression rate of focal segmental glomerulosclerosis in Iranian children
Gheissari, A., Salehi, M., Dastjerdi, S.B., Jahangiri, M., Hooman, N., Otookesh, H., Merikhipour, A.,Naeini, A.E.
Nephrology. 2008; 13(8): 708-711
[Pubmed]
3 Angiotensin I-Converting enzyme insertion-related genotypes and allele are associated with higher susceptibility of endometriosis and leiomyoma
Hsieh YY, Lee CC, Chang CC, et al.
MOLECULAR REPRODUCTION AND DEVELOPMENT. 2007; 74 (7): 808-814
[Pubmed]
4 High prevalence of ACE DD genotype among north Indian end stage renal disease patients
Tripathi, G., Dharmani, P., Khan, F., Sharma, R.K., Pandirikkal, V., Agrawal, S.
BMC Nephrology. 2006; 7(Art no 15)
[Pubmed]
5 Angiotensin-converting enzyme gene polymorphism in children with idiopathic nephrotic syndrome
Tsai IJ, Yang YH, Lin YH, et al.
AMERICAN JOURNAL OF NEPHROLOGY. 2006; 26 (2): 157-162
[Pubmed]
6 Angiotensin I-converting enzyme ACE 2350*G and ACE-240*T-related genotypes and alleles are associated with higher susceptibility to endometriosis
Hsieh YY, Chang CC, Tsai FJ, et al.
MOLECULAR HUMAN REPRODUCTION. 2005; 11 (1): 11-14
[Pubmed]
7 ACE gene insertion/deletion polymorphism in childhood idiopathic nephrotic syndrome
Serdaroglu E, Mir S, Berdeli A, Aksu N, Bak M
PEDIATRIC NEPHROLOGY. 2005; 20 (12): 1738-1743
[Pubmed]
8 Racial and Ethnic Differences in the Incidence and Progression of Focal Segmental Glomerulosclerosis in Children
Andreoli, S.P.
Advances in Renal Replacement. 2004; 11(1): 105-109
[Pubmed]



 

Top
Print this article  Email this article
Previous article Next article
Online since 12th February '04
© 2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow