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 ORIGINAL ARTICLE
Year : 2008  |  Volume : 54  |  Issue : 1  |  Page : 4-6

Detection of Rh antibodies using two low ionic diluents: Extension of the incubation time and the number of Rh antibodies detected


Department of Laboratory Medicine, Al-Azhar University - Gaza, Gaza, Palestine. 16/46 Omar Al-Mokhtar Street, AL-Rimal, Gaza, Palestine

Correspondence Address:
Y A Skaik
Department of Laboratory Medicine, Al-Azhar University - Gaza, Gaza, Palestine. 16/46 Omar Al-Mokhtar Street, AL-Rimal, Gaza, Palestine

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0022-3859.39181

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Background: Low ionic strength solution (LISS) is used to increase the rate of association of antibody to the corresponding antigen during antibody detection tests. A number of LISSs are available on the market. Aims: The efficiency of two commercial low ionic diluents, DiaMed ID-CellStab and Inverclyde LISS were assessed using the DiaMed-ID LISS Coombs microtube column system and an incubation time varying from 15 to 35 min. Materials and Methods: One-hundred samples containing five Rh antibodies (anti-D, anti-C, anti-E, anti-c and anti-e) were tested against commercial red cells using the two low ionic diluents after 15, 25 and 35 min. Results: The Inverclyde LISS detected 91, 95 and 96% of the Rh antibodies compared to 78, 79 and 83% for ID-CellStab after 15, 25 and 35 min incubation time, respectively, for both methods. Conclusions: The Inverclyde LISS is a more suitable and efficient diluent than ID-CellStab for the detection of Rh antibodies. The sensitivity of Rh antibody detection increased for the both methods as the incubation time increased.






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Online since 12th February '04
2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow