Journal of Postgraduate Medicine
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 BRIEF REPORT
Year : 2016  |  Volume : 62  |  Issue : 2  |  Page : 105-108

Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India


Department of Virology and Immunology, Haffkine Institute for Training, Research and Testing, Mumbai, Maharashtra, India

Correspondence Address:
P Charan
Department of Virology and Immunology, Haffkine Institute for Training, Research and Testing, Mumbai, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0022-3859.175006

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Context: Rabies poses a serious public health concern in developing countries such as India. Aims: The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India. Materials and Methods: Nucleoprotein gene from eight (of total 20 suspected samples) rabies cases that tested positive for rabies antigen using reverse transcriptase-polymerase chain reaction (RT-PCR) were sequenced. Results: Sequence analysis using basic local alignment search tool (BLAST) and multiple sequence alignment (MSA) and phylogenetic analysis showed similarity to previously reported sequences from India and those of Arctic lineages. Conclusions: The circulating RABV strains in Maharashtra, India show genetic relatedness to RABV strains reported from Indo-Arctic lineages and India-South and Japan.






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Online since 12th February '04
2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow