Journal of Postgraduate Medicine
 Open access journal indexed with Index Medicus & ISI's SCI  
Users online: 737  
Home | Subscribe | Feedback | Login 
About Latest Articles Back-Issues Article Submission Resources Sections Etcetera Contact
 
  NAVIGATE Here 
  Search
 
  
 RESOURCE Links
 ::  Similar in PUBMED
 ::  Search Pubmed for
 ::  Search in Google Scholar for
 ::Related articles
 ::  Article in PDF (407 KB)
 ::  Citation Manager
 ::  Access Statistics
 ::  Reader Comments
 ::  Email Alert *
 ::  Add to My List *
* Registration required (free) 

  IN THIS Article
 ::  Abstract
 :: Introduction
 ::  Materials and Me...
 :: Results
 :: Discussion
 :: Conclusion
 ::  References
 ::  Article Figures
 ::  Article Tables

 Article Access Statistics
    Viewed1602    
    Printed80    
    Emailed0    
    PDF Downloaded64    
    Comments [Add]    

Recommend this journal


 


 
  Table of Contents     
ORIGINAL ARTICLE
Year : 2017  |  Volume : 63  |  Issue : 2  |  Page : 92-95

Do high sensitivity C-reactive protein and serum interleukin-6 levels correlate with disease activity in systemic lupus erythematosuspatients?


1 Department of Clinical and Experimental Immunology, National Institute of Immunohaematology, Indian Council of Medical Research, Mumbai, Maharashtra, India
2 Department of Medicine, King Edward Memorial Hospital, Mumbai, Maharashtra, India

Date of Submission13-Mar-2016
Date of Decision25-Jun-2016
Date of Acceptance29-Jun-2016
Date of Web Publication10-Apr-2017

Correspondence Address:
V D Pradhan
Department of Clinical and Experimental Immunology, National Institute of Immunohaematology, Indian Council of Medical Research, Mumbai, Maharashtra
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0022-3859.188550

Rights and Permissions


 :: Abstract 

Introduction: Systemic Lupus Erythematosus (SLE) is an inflammatory autoimmune disease where an interplay between acute phase proteins and cytokines are involved in disease activation. Aim and Objectives: This case control study was performed to investigate interrelationship between high sensitivity C-reactive proteins (hs-CRP), Interleukin-6 (IL-6) levels and disease activity among SLE patients. Materials and Methods: One hundred forty one clinically diagnosed SLE cases were included and disease activity was noted by SLE Disease Activity Index (SLEDAI). Serum IL-6 levels were measure by cytokine multiplex assay. Serum hs-CRP, C3 and C4 levels were measure by nephelometer. The Pearson correlation test was used for correlation between hs-CRP, Il-6 and SLEDAI. Results: Based on SLEDAI, 126 patients (89.4 %) had active disease and 15 patients (10.6%) had inactive disease. Mean hs-CRP levels in SLE patients were significantly higher (12.1+ 11.5 mg/L) than controls (2.41+ 1.37 mg/L) (P < 0.0001). Hs-CRP levels among active SLE were significantly higher (13.5+ 11.4 mg/L) as compared with inactive SLE (4.4 + 2.9 mg/L) (P=0.0002). Similarly, IL-6 levels in SLE patients were significantly higher among active SLE (26.9 + 15.5 pg/ml) as compared with inactive SLE (13.9+ 10.2 pg/ml) (P=0.0001). An inverse correlation between Il-6 and hemoglobin levels between active and inactive SLE was noted (r=-0.46, P <0.0001). Conclusion: This study suggests a good correlation between hs-CRP, IL-6 and SLE disease activity indicating their direct involvement in inflammatory conditions associated with disease.


Keywords: High sensitivity C-reactive protein levels, interleukin-6 levels, systemic lupus erythematosus, systemic lupus erythematosus disease activity index


How to cite this article:
Umare V, Nadkarni A, Nadkar M, Rajadhyksha A, Khadilkar P, Ghosh K, Pradhan V D. Do high sensitivity C-reactive protein and serum interleukin-6 levels correlate with disease activity in systemic lupus erythematosuspatients?. J Postgrad Med 2017;63:92-5

How to cite this URL:
Umare V, Nadkarni A, Nadkar M, Rajadhyksha A, Khadilkar P, Ghosh K, Pradhan V D. Do high sensitivity C-reactive protein and serum interleukin-6 levels correlate with disease activity in systemic lupus erythematosuspatients?. J Postgrad Med [serial online] 2017 [cited 2017 Oct 20];63:92-5. Available from: http://www.jpgmonline.com/text.asp?2017/63/2/92/188550



 :: Introduction Top


Systemic lupus erythematosus (SLE) is a multi-factorial chronic inflammatory autoimmune disease characterized by the production of a wide spectrum of autoantibodies and immune complex deposition leading to multiple organ damage. An inflammatory response is mediated through the influx of proinflammatory cytokines. These cytokines further induce production of various acute-phase reactant proteins such as high sensitivity C-reactive proteins (hs-CRPs), lectin proteins, hepcidin, etc., and these proteins are established as inflammatory markers.[1],[2] CRP is one of the primary acute phase proteins, mainly synthesized by hepatocytes in response to proinflammatory cytokines such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and IL-1 βeta (IL-1 β). Raised hs-CRP levels have been reported in response to a variety of cellular abnormalities such as tissue inflammation, infection, cardiovascular disease, and thromboembolism.[3],[4]

In SLE, hs-CRP concentration rises sharply during acute inflammatory conditions. Positive correlations have been reported between hs-CRP levels and SLE disease activity index (SLEDAI).[5],[6] Several studies have reported significance of various proinflammatory cytokines such as IL-6, TNF-α, and IL-1 β in SLE patients indicating their pathophysiological role mainly in active SLE patients.[7],[8] There is a paucity of data regarding the relationship among inflammatory cytokines like IL-6, raised CRP and various risk factors contributing to SLE disease severity. In this study, the possible correlation between CRP and serum IL-6 levels with disease activity among Indian SLE patients was evaluated.


 :: Materials and Methods Top


Study population

One hundred and forty-one patients (131 females and 10 males; mean age at evaluation 27 ± 11 years, range 14–54 years) fulfilling the American College of Rheumatology classification criteria for SLE were recruited.[9] This retrospective case-control study was conducted over a period of 4 years (2010–2013). Pregnant and postmenopausal women, smokers, patients with diabetes and with significant hyperlipemia were excluded. One hundred and fifty age and sex-matched healthy individuals were included as controls. The study was approved by the Institutional Ethics Committee and written consent was obtained from all patients and controls. Disease severity was evaluated by calculating the SLEDAI score.[10] A score equal to or more than 10 was considered to be active.[11]

Laboratory investigations

Venous blood samples from all patients and healthy controls were collected for investigating hemoglobin levels, serum CRPs, and IL-6 levels. Sera were stored in aliquots at −80°C until tested. Twenty-four hour urine was collected for urinalysis. Serum hs-CRP levels, complement component (C3, C4) levels were measured using a Nephelometer (BN Prospec, Dade Behring, Germany). Serum cytokine levels were detected by bead-based MILLIPLEX MAP technology (Millipore Corporation, Billerica, MA, USA.) in all patients and healthy controls.

Statistical analysis

Statistical analyses were performed using GraphPad Prism version 5.03 (GraphPad Prism Software Inc., California, USA.) for Windows. Comparisons of serum levels of hs-CRP and IL-6 in SLE patients with or without active disease were performed using nonparametric Mann–Whitney U-test. Means between two groups were analyzed using two-tailed unpaired t-test. Fischer's exact test was used to determine an association between laboratory investigations, clinical manifestations, and the presence of autoantibodies in SLE patients. The Pearson's correlation test was used to analyze correlations between various laboratory measures and SLEDAI scores. The value of P ≤ 0.05 was considered statistically significant.


 :: Results Top


The mean age of SLE patients at the time of evaluation was 29.4 ± 9.8 years where mean disease duration was 2.7 ± 1.9 years. Mean SLEDAI score was found to be 16.9 ± 7.6. Of 141 SLE patients, 69 patients (48.9%) were lupus nephritis (LN) and 72 patients (51.1%) were without renal involvement (non-LN). Based on SLEDAI, 126 patients (116 females and 10 males) (89.4%) were categorized as active SLE (SLEDAI > 10) and 15 patients (all females) (10.6%) as an inactive SLE (SLEDAI < 10). Mean CRP levels, urine proteins and serum creatinine levels were significantly higher in active SLE than inactive SLE (P < 0.01), whereas mean hemoglobin level was significantly lower among active SLE patients (P = 0.0029) [Table 1]. Reduced levels of C3 alone were significantly higher in active SLE as compared with inactive SLE (P = 0.0010). Among active SLE, 57.1% showed arthritis and 52% had renal involvement. Statistically significant difference was noted for arthritis and renal manifestations when active and inactive SLE groups were compared (P = 0.0302, P = 0.0266, respectively). Similarly, hematological disorders like low Hb, leukopenia and thrombocytopenia were higher as in active SLE than inactive SLE (44.4% vs. 13.3%).
Table 1: Laboratory investigations done in systemic lupus erythematosus patients (n=172)

Click here to view


The mean hs-CRP levels among SLE patients were significantly higher (12.6 ± 20.9 mg/L) as compared to healthy individuals (3.41 ± 1.37 mg/L). Among SLE patients, mean hs-CRP levels were significantly higher in active SLE (13.6 ± 21.9 mg/L) as compared to inactive SLE (4.8 ± 3.8 mg/L) (P = 0.0029). When hs-CRP levels with SLEDAI were compared, a positive correlation (r = 0.298, P = 0.0003) was observed [Figure 1]a. Mean serum IL-6 levels among SLE patients were 68.1 ± 68.0 pg/mL, which was 4.8 times higher than healthy controls (14.16 ± 5.83 pg/mL) and this difference was statistically significant (P < 0.0001). Mean IL-6 levels were significantly higher among active SLE (74.9 ± 68.5 pg/mL) as compared to inactive SLE (19.0 ± 3.8 pg/mL, P = 0.0036). IL-6 levels were significantly elevated among LN patients (80.4 ± 61.0 pg/mL) as compared to non-LN patients (57.9 ± 72.7 pg/mL, P = 0.0488). When IL-6 levels were compared with SLEDAI positive correlation was observed (r = 0.271, P = 0.001) [Figure 1]b.
Figure 1: Correlation between levels of (a) hs-CRP and (b) IL-6 with SLEDAI index among SLE patients (n = 141). Hs-CRP: high-sensitivity C-reactive protein; IL-6: Interleukin-6; SLEDAI: SLE Disease Activity Index; r, Pearson's correlation coefficient

Click here to view


A significant positive correlation was also noted between IL-6 levels and hs-CRP levels in SLE patients (r = 0.411, P < 0.0001). An inverse correlation between serum IL-6 and hemoglobin levels was seen among SLE patients (r = −0.214, P = 0.0106). Mean IL-6 levels ware higher (62.2 ± 49.1 pg/mL) in patients with anemia (Hb ≤ 12.0 g/dl) than those without anemia (24.6 ± 23.1 pg/mL). This difference was found to be statistically significant (P = 0.0006).


 :: Discussion Top


CRP is an inflammatory marker produced in response to IL-6 stimulation by hepatocytes and adipocytes.[12] hs-CRP levels have been found elevated in SLE but its correlation with SLE disease activity was reported to be controversial.[13],[14] Bertoli et al. had reported that SLE patients from Hispanic, African American, and Caucasian populations show a significant association of hs-CRP levels with the disease activity. It was reported that elevated hs-CRP levels are associated with multiple organ damage, including renal, cardiovascular, and musculoskeletal manifestations in SLE.[6] Lee et al. also had reported an association of hs-CRP levels with the disease activity and organ damage to the musculoskeletal system in their multiethnic cohort study including Asian SLE patients.[5]

In this study, significantly higher levels of hs-CRP were noted in active SLE patients as compared to inactive as well as healthy controls (P < 0.01). Pathogenic role and statistically significant association between IL-6 levels and disease activity was also reported in SLE patients by Sabry et al. in Egyptian SLE patients and Chun et al. in Korean population where higher serum levels of IL-6 in active SLE and its association with disease activity had been reported.[15],[16] In our study, a significantly higher level of both hs-CRP and IL-6 in clinically active SLE (P < 0.05) with serositis was noted which was similar to study reported by Spronk et al.[17] Enocsson et al. also had reported increased CRP levels among SLE patients compared to controls, without any association with IL-6 levels and SLEDAI.[18] There are a few reports suggesting cardiovascular risk in active SLE patients with higher hs-CRP levels and an increased cardiovascular morbidity.[3],[4],[5],[19] The limitations of our study were that in this study the impact of measurement of immune parameters such as hs-CRP and IL-6 during remission or relapses could not be evaluated which needs to be evaluated in details in patients with varied disease activity.

The relationship between anemia and inflammatory conditions in autoimmune disorders has been well documented.[20] Ripley et al. had reported low hemoglobin levels with the increased serum IL-6 which was similar to our study where an inverse correlation between IL-6 and hemoglobin levels was found.[21] This study supports the finding that a triangular measurement of hs-CRP, IL-6 and disease activity will be helpful in assessing the disease severity among active SLE patients.


 :: Conclusion Top


This study suggests a good correlation between hs-CRP, IL-6 and SLE disease activity indicating their direct involvement in inflammatory conditions associated with disease.

Financial support and sponsorship

This work was supported by a grant from Council of Scientific and Industrial Research, Government of India (CSIR(27 (0276)/12/EMR-II)). We are grateful to Indian Council of Medical Research, Government of India, for providing us the research facilities.

Conflicts of interest

There are no conflicts of interest.

 
 :: References Top

1.
Arbuckle MR, McClain MT, Rubertone MV, Scofield RH, Dennis GJ, James JA, et al. Development of autoantibodies before the clinical onset of systemic lupus erythematosus. N Engl J Med 2003;349:1526-33.  Back to cited text no. 1
    
2.
Baumann H, Gauldie J. Regulation of hepatic acute phase plasma protein genes by hepatocyte stimulating factors and other mediators of inflammation. Mol Biol Med 1990;7:147-59.  Back to cited text no. 2
    
3.
Barnes EV, Narain S, Naranjo A, Shuster J, Segal MS, Sobel ES, et al. High sensitivity C-reactive protein in systemic lupus erythematosus: Relation to disease activity, clinical presentation and implications for cardiovascular risk. Lupus 2005;14:576-82.  Back to cited text no. 3
    
4.
Mok CC, Birmingham DJ, Ho LY, Hebert LA, Rovin BH. High-sensitivity C-reactive protein, disease activity, and cardiovascular risk factors in systemic lupus erythematosus. Arthritis Care Res (Hoboken) 2013;65:441-7.  Back to cited text no. 4
    
5.
Lee SS, Singh S, Link K, Petri M. High-sensitivity C-reactive protein as an associate of clinical subsets and organ damage in systemic lupus erythematosus. Semin Arthritis Rheum 2008;38:41-54.  Back to cited text no. 5
    
6.
Bertoli AM, Vilá LM, Reveille JD, Alarcón GS; LUMINA Study Group. Systemic lupus erythematosus in a multiethnic US cohort (LUMINA): LXI. Value of C-reactive protein as a marker of disease activity and damage. J Rheumatol 2008;35:2355-8.  Back to cited text no. 6
    
7.
Davas EM, Tsirogianni A, Kappou I, Karamitsos D, Economidou I, Dantis PC. Serum IL-6, TNFalpha, p55 srTNFalpha, p75srTNFalpha, srIL-2alpha levels and disease activity in systemic lupus erythematosus. Clin Rheumatol 1999;18:17-22.  Back to cited text no. 7
    
8.
McCarthy EM, Smith S, Lee RZ, Cunnane G, Doran MF, Donnelly S, et al. The association of cytokines with disease activity and damage scores in systemic lupus erythematosus patients. Rheumatology (Oxford) 2014;53:1586-94.  Back to cited text no. 8
    
9.
Hochberg MC. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1997;40:1725.  Back to cited text no. 9
    
10.
Urowitz MB, Gladman DD. Measures of disease activity and damage in SLE. Baillieres Clin Rheumatol 1998;12:405-13.  Back to cited text no. 10
    
11.
Rana A, Minz RW, Aggarwal R, Anand S, Pasricha N, Singh S. Gene expression of cytokines (TNF-α, IFN-γ), serum profiles of IL-17 and IL-23 in paediatric systemic lupus erythematosus. Lupus 2012;21:1105-12.  Back to cited text no. 11
    
12.
Lau DC, Dhillon B, Yan H, Szmitko PE, Verma S. Adipokines: Molecular links between obesity and atheroslcerosis. Am J Physiol Heart Circ Physiol 2005;288:H2031-41.  Back to cited text no. 12
    
13.
Amezcua-Guerra LM, Springall R, Arrieta-Alvarado AA, Rodríguez V, Rivera-Martinez E, Castillo-Martinez D, et al. C-reactive protein and complement components but not other acute-phase reactants discriminate between clinical subsets and organ damage in systemic lupus erythematosus. Clin Lab 2011;57:607-13.  Back to cited text no. 13
    
14.
Rezaieyazdi Z, Sahebari M, Hatef MR, Abbasi B, Rafatpanah H, Afshari JT, et al. Is there any correlation between high sensitive CRP and disease activity in systemic lupus erythematosus? Lupus 2011;20:1494-500.  Back to cited text no. 14
    
15.
Sabry A, Sheashaa H, El-Husseini A, Mahmoud K, Eldahshan KF, George SK, et al. Proinflammatory cytokines (TNF-alpha and IL-6) in Egyptian patients with SLE: Its correlation with disease activity. Cytokine 2006;35:148-53.  Back to cited text no. 15
    
16.
Chun HY, Chung JW, Kim HA, Yun JM, Jeon JY, Ye YM, et al. Cytokine IL-6 and IL-10 as biomarkers in systemic lupus erythematosus. J Clin Immunol 2007;27:461-6.  Back to cited text no. 16
    
17.
Spronk PE, ter Borg EJ, Limburg PC, Kallenberg CG. Plasma concentration of IL-6 in systemic lupus erythematosus; an indicator of disease activity? Clin Exp Immunol 1992;90:106-10.  Back to cited text no. 17
    
18.
Enocsson H, Sjöwall C, Skogh T, Eloranta ML, Rönnblom L, Wetterö J. Interferon-alpha mediates suppression of C-reactive protein: Explanation for muted C-reactive protein response in lupus flares? Arthritis Rheum 2009;60:3755-60.  Back to cited text no. 18
    
19.
Nikpour M, Gladman DD, Ibañez D, Urowitz MB. Variability and correlates of high sensitivity C-reactive protein in systemic lupus erythematosus. Lupus 2009;18:966-73.  Back to cited text no. 19
    
20.
Weiss G, Goodnough LT. Anemia of chronic disease. N Engl J Med 2005;352:1011-23.  Back to cited text no. 20
    
21.
Ripley BJ, Goncalves B, Isenberg DA, Latchman DS, Rahman A. Raised levels of interleukin 6 in systemic lupus erythematosus correlate with anaemia. Ann Rheum Dis 2005;64:849-53.  Back to cited text no. 21
    


    Figures

  [Figure 1]
 
 
    Tables

  [Table 1]



 

Top
Print this article  Email this article
 
Online since 12th February '04
2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow