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Figure 5 :Polymerase chain reaction (PCR) analysis of EWS-WT1 translocation using EWS and WT1 primers. Reactions were subjected to electrophoresis on 10% polyacrylamide gel. Lane 1: the DNA size markers in base pairs (bp); Lane 2: PCR run was performed with cDNA from patient sample; Lane 3: Positive control DNA (pTZ57R/T-EWSWT1- 259bp); Lane 4: Positive control DNA (pTZ57R/T-EWS-WT1- 268bp); Lane 5: PCR amplification without DNA template to rule out contamination

Figure 5 :Polymerase chain reaction (PCR) analysis of EWS-WT1 translocation using EWS and WT1 primers. Reactions were subjected to electrophoresis on 10% polyacrylamide gel. Lane 1: the DNA size
markers in base pairs (bp); Lane 2: PCR run was performed with cDNA from patient sample; Lane 3: Positive control DNA (pTZ57R/T-EWSWT1- 259bp); Lane 4: Positive control DNA (pTZ57R/T-EWS-WT1- 268bp); Lane 5: PCR amplification without DNA template to rule out contamination