Journal of Postgraduate Medicine
 Open access journal indexed with Index Medicus & ISI's SCI  
Users online: 1842  
Home | Subscribe | Feedback | Login 
About Latest Articles Back-Issues Articlesmenu-bullet Search Instructions Online Submission Subscribe Etcetera Contact
 :: Next article
 :: Previous article 
 :: Table of Contents
 ::  Similar in PUBMED
 ::  Search Pubmed for
 ::  Search in Google Scholar for
 ::Related articles
 ::  Article in PDF (298 KB)
 ::  Citation Manager
 ::  Access Statistics
 ::  Reader Comments
 ::  Email Alert *
 ::  Add to My List *
* Registration required (free) 

  IN THIS Article
 ::  Abstract
 ::  Conclusion
 ::  References
 ::  Article Tables

 Article Access Statistics
    PDF Downloaded1354    
    Comments [Add]    
    Cited by others 12    

Recommend this journal


Year : 2006  |  Volume : 52  |  Issue : 3  |  Page : 210-217

Chemokines and chemokine receptors in HIV infection: Role in pathogenesis and therapeutics

Department of Internal Medicine, PGIMER, Chandigarh - 160 012, Punjab, India

Correspondence Address:
A Wanchu
Department of Internal Medicine, PGIMER, Chandigarh - 160 012, Punjab
Login to access the Email id

Source of Support: None, Conflict of Interest: None

PMID: 16855325

Rights and PermissionsRights and Permissions

 :: Abstract 

Chemokines are known to function as regulatory molecules in leukocyte maturation, traffic, homing of lymphocytes and in the development of lymphoid tissues. Besides these functions in the immune system, certain chemokines and their receptors are involved in HIV pathogenesis. In order to infect a target cell, the HIV envelope glycoprotein gp120 has to interact with the cellular receptor CD-4 and co-receptor, CC or CXC chemokine receptors. Genetic findings have yielded major insights into the in vivo roles of individual co-receptors and their ligands in providing resistance to HIV infection. Mutations in chemokine receptor genes are associated with protection against HIV infections and also involved in delayed progression to AIDS in infected individuals. Blocking of chemokine receptors interrupts HIV infection in vitro and this offers new options for therapeutic strategies. Approaches have been made to study the CCR-5 inhibitors as antiviral therapies and possibly as components of a topical microbicide to prevent HIV-1 sexual transmission. Immune strategies aimed at generating anti-CCR-5 antibodies at the level of the genital mucosa might be feasible and represent a strategy to induce mucosal HIV- protective immunity. It also remains to be seen how these types of agents will act in synergy with existing HIV-1 targeted anti viral, or those currently in developments. Beyond providing new perspectives in fundamental aspects of the HIV-1 transmission and pathogenesis, chemokines and their receptors suggest new areas for developing novel therapeutic and preventive strategies against HIV infections. Studies in this review were identified through a search for relevant literature in the pubmed database of the national library of medicine. In this review, some developments in chemokine research with particular focus on their roles in HIV pathogenesis, resistance and therapeutic applications have been discussed.

Keywords: HIV-1, chemokine, host factors, pathogenesis, anti HIV therapy

How to cite this article:
Suresh P, Wanchu A. Chemokines and chemokine receptors in HIV infection: Role in pathogenesis and therapeutics. J Postgrad Med 2006;52:210-7

How to cite this URL:
Suresh P, Wanchu A. Chemokines and chemokine receptors in HIV infection: Role in pathogenesis and therapeutics. J Postgrad Med [serial online] 2006 [cited 2023 Jun 5];52:210-7. Available from:

Chemokines are a superfamily of secreted proteins that function in leukocyte trafficking, recruiting and recirculation. They also play a critical role in many pathophysiological processes such as allergic responses, infectious and autoimmune diseases, angiogenesis, inflammation, tumor growth and hematopoietic development. They are secreted by a variety of cells in the immune system. All chemokines signal through seven transmembrane domain G-protein coupled receptors and many of these receptors exhibit promiscuous binding properties whereby several different chemokines can signal through the same receptor. To date, more than 40 distinct chemokines have been well characterized, some of which are listed in [Table - 1]. Discoveries over the past few years have defined a close relationship between chemokines and HIV infection. Apart from their well established role in blocking viral entry by binding to their receptors, chemokines have additional roles in HIV pathogenesis. For several years, it has been known that CD 8+ T cells secrete factors that suppress HIV-1 replication in CD4 + T cells.[1],[2] The nature of these factors remained unknown until Cocchi et al in 1995 showed that the β chemokines MIP-lα (macrophage inflammatory protein 1α), MIP-Iβ (macrophage inflammatory protein 1β ) and RANTES (regulated on activation, normal T expressed and secreted) contributed to the CD 8+ cell suppressive effect.[3] A breakthrough in our understanding of HIV pathogenesis was the identification of a chemokine receptor like molecule in 1996, called LESTR, subsequently designated as CXCR-4, as a necessary co-receptor for X 4 variants of HIV entry into cells.[4],[5] In the absence of the second receptor, HIV-1 can bind to its target cells (via CD-4), but the fusion process is not initiated. The identification of the beta chemokine receptor (CC) CKR-5 (later renamed as CCR-5) as the primary co-receptor for macrophage-tropic, non-syncytium inducing (NSI) strains of HIV-1 was also reported in 1996, almost simultaneously by five independent groups of researchers.[6],[7],[8],[9],[10]

Chemokine receptors as co-receptors for HIV cell entry

HIV envelope proteins gp 120 and gp 41 mediate binding of virus to the host cell surface through high affinity interaction with CD-4, the primary virus receptor. Subsequent interaction with the appropriate chemokine receptor CCR-5 or CXCR-4 triggers the final conformational changes in env , resulting in fusion between the viral and cellular membrane.[11],[12],[13] The observation that HIV-1 isolates differ in their ability to use two major co-receptors (CCR-5 and CXCR-4) has provided a key to understand the physiological basis of the biological variability of HIV-1. Different HIV-1 variants use either CCR-5 or CXCR-4 or both. According to the terminology based on co-receptor usage, CXCR-4 using viruses are termed X 4 and CCR5-using viruses, R 5. Viruses that were previously defined as SI which use both CXCR-4 and CCR-5 receptors are termed X 4 R 5 (R 3) viruses.[14],[15] Using transfected cell lines other chemokine receptors such as CCR-3, CCR-2, CCR-8, CCR-9, STRL-33, Gpr 15, Gpr 1, APJ, Chem R 23 and CX 3 CR1 were identified and shown to be used by certain HIV strains for cell entry.[9],[10],[16],[17],[18] Recently a promiscuous CC chemokine receptor, D6, has been found that can function as a co-receptor for various primary dual-tropic isolates of HIV-1 and HIV-2.[19] Despite this broad spectrum of potentially available co-receptors, CCR-5 and CXCR-4 appear to be the most relevant co-receptors for HIV-1 in vivo. The host's natural ligands for these co-receptors are relevant because they might interfere with HIV entry into target cells by interfering with viral binding to the receptor or by down regulating the receptor. CCR-5 binds to RANTES, MIP-1α and MIP-1β , which are the members of the β chemokine family whereas CXCR4 binds to a member of a chemokine family, stromal cell derived factor 1 (SDF-1). CCR2 binds to monocyte chemotactic protein-1 (MCP-1) through MCP-5 and CCR-3 binds to MCP-3, MCP-4 and eotaxin1 and 2. CCR-5 using chemokine can block R 5 strains of HIV, whereas SDF-1 blocks X 4 strains. CCR 5 and CXCR-4 receptor has been expressed on a variety of cells and tissues. Chemokine receptor expression in a specific cellular type might be constitutive or inducible.[20]

Chemokines and HIV pathogenesis

Immunologic or genetic alterations that affect chemokine levels might have an impact on the susceptibility to HIV infection or the rate of progression once the infection is established. Inhibition of HIV entry by chemokines depends on two possible mechanisms: a steric effect that consists of the competitive blockade of viral entry by direct union of ligand to its receptor, or through the internalization of the receptor after chemokine binding. Alternatively, chemokine receptor dimerization mediated through binding of chemokine could account for inhibition of HIV entry and virus replication.[21],[22],[23] An in vitro study by the Multicenter AIDS cohort study (MACS) showed that AIDS free status and higher CD 4 counts correlate with higher CCR-5 ligands release from peripheral blood mononuclear cells after activation in vitro by HIV antigen.[24] In addition, cells from individuals who are exposed but seronegative to HIV infection release significantly higher levels of chemokines than seronegative controls and HIV positive subjects, even in the absence of an in vitro stimulus.[24] These studies suggest that chemokine release is a very early response to the exposure to HIV. It is also reported that high levels of CCR-5 using chemokines are associated with slower disease progression.[25] An in vitro study which assessed the susceptibility to HIV infection of CD-4 + cells from HEPS (highly exposed persistently seronegative) individuals in a discordant cohort showed that CD-4 + lymphocytes from HEPS individuals were less susceptible to HIV infection by R 5 strains of HIV. In addition, CD-4 + lymphocytes of these HEPS subjects produced significantly higher levels of RANTES, MIP-1α and MIP-1β upon stimulation with phytohemagglutinin (PHA) and enhanced chemokine production was noticed against stimulation with HIV gag peptide.[26] Other studies have also reported increased β chemokine levels from serum samples in a group of Chinese HEPS individuals and enhanced production of MIP-1β by CD-8 + T cells from HEPS subjects in a discordant couple cohort.[27],[28] Other studies have shown that CD-8 + T cells from asymptomatic individuals produce higher levels of MIP-1α and MIP-1β , but not RANTES, than CD-8 T cells from healthy donors or patients with rapid progression.[29],[30],[31],[32],[33]

Some other studies have found no association between chemokine production and HIV resistance in HEPS individuals and also between long term nonprogressors and rapid progressors.[34],[35],[36],[37] Others have even suggested that RANTES, MIP-1a and MIP-1β may upregulate replication of HIV in macrophages and monocytes by recruiting activated target cells.[38],[39],[40] Other chemokines that affect HIV replication, although they are not involved in viral entry, are interferon-g-inducible protein 10 (IP-10/CXCL-10) and monocyte chemotactic protein (MCP)-1.[41],[42],[43] These chemokines have been detected in the cerebrospinal fluid of HIV infected individuals. Another chemokine that may affect HIV infection is IL-8 (CXCL-8). Increased levels of circulating IL-8 have been detected in HIV infected individuals.[44] Upregulation of chemokine expression by cells of the immune system would have local and systemic effects that contribute significantly to the pathogenesis of HIV infection.[45],[46] The exact role of chemokines in HIV-1 pathogenesis remains obscure, probably due to the fact that multiple chemokines may have different effects on viral replication and pathogenesis, or their effect might be compromised by viral factors.

CCR-5 gene mutations

The observation that chemokine receptors are used by HIV as co-receptors for cellular entry led to the discovery of genetic host factors that can affect susceptibility to infection with HIV or the rate of progression to disease once infection is established. In early HIV infection, a vast majority of HIV isolates use the CC chemokine receptor, referred to as CCR-5, which in blood is expressed on a variety of cells including CD-4, CD-8, memory and activated T cells. Individuals homozygous for a 32 base pair deletion in their CCR-5 gene (referred to as CCR5-D32), are almost completely resistant to HIV infection.[47] CCR5-D32 was the best characterized genetic trait, identified in 1996.[50],[51] The mutation is a 32 base pair deletion corresponding to the second extracellular loop of the 7-transmembrane G-coupled protein receptor in the CCR-5 gene, which causes a frame shift, leading to a premature termination of translation and the resulted protein encoded by this mutant lacks three transmembrane segment of the receptor. Such a truncated protein is nonfunctional.[47] In epidemiological studies, the allelic frequency of the CCR-5 gene deletion was 10-20% among caucasians, particularly amongst those of Northern European descent with 1% homozygosity. This mutation is extremely rare in African and Asian population.[48],[51] Individuals homozygous for the CCR5-D-32 allele do not express any of the CCR-5 receptor on their cell surfaces and in turn, they are largely resistant to infection by HIV-1. Different studies have shown that CCR-5-D-32 mutation is extremely protective against HIV-1 infection, although they can still be infected with X 4 strains of HIV, which use the CXCR-4 co-receptor for cell entry.[48],[49],[50],[51] However, this protection is not complete, as a few individuals homozygous for this deletion were infected and the virus that was isolated from these individuals was X 4 type.[52],[53] Some other studies have reported the presence of dual tropic R5X4 HIV strains in two individuals homozygous for CCR-5-D-32 allele.[54],[55] Studies of CCR-5-D-32 mutation in exposed but uninfected individuals have revealed that a small proportion of them were homozygous for this mutation.[47],[56],[57],[59] Some studies have found that CCR-5-D-32 heterozygosity was associated with delayed progression to AIDS in infected individuals and also reported that frequency of heterozygosity was significantly greater in long term non-progressors than in progressors and rapid progressors.[47],[48],[49],[50],[51],[57],[58],[59],[60],[61] The mechanism of protection is not clear and it is believed that CCR-5 expression may be altered in these individuals. In our own cohort of such individuals, we were unable to detect any deletion.

A point mutation in the coding region of CCR-5 gene conferring in vitro and in vivo resistance to R 5 virus has been identified.[62] The mutation is characterized by an open reading frame single T to A base pair transversion at nucleotide 303 which indicates a cysteine to stop codon change in the first extracellular loop of the chemokine receptor protein at amino acid 101.[63],[64] This mutation when found in the compound heterozygous state with CCR5-D-32 was associated with increased protection. However, this allele is very rare with an allelic frequency less than 1 percent.[64]

CCR-5 promoter and regulatory gene polymorphisms

Several genetic polymorphisms have been identified within the CCR5 regulatory or promoter region that might affect HIV transmission or disease progression, possibly through its effect upon levels of CCR5 expression.[58],[64],[65],[66],[67]

CCR-5 59029 G: This is an A/G polymorphism at base pair 59029 in the CCR-5 promoter. HIV infected persons who are homozygous for allele 59029 G within the CCR-5 promoter regulatory region progress to AIDS more slowly than those who are homozygous for allele 59029 A.[65] Another study shows an association between CCR-5 promoter polymorphisms and long-term asymptomatic HIV-1 infection, with individuals lacking the CCR-5 59029A/CCR5 59353C homozygous genotype likely to progress more slowly towards AIDS and/or death.[66] Yet another point mutation at position 59402 results in an A/G substitution and homozygosity of this allele is associated with reduced perinatal transmission. This mutation affects the CCR-5 expression on CD 4 + T cells.[67] Other promoter haplotypes may lead to more rapid disease progression.[58] Homozygosity of another polymorphism known as CCR-5 59356T has been strongly associated with increased rate of perinatal transmission and this polymorphism occurs more frequently in black persons than white.[67]

CCR-2b 64I mutation

The chemokine receptor CCR-2b is identified as a minor HIV-1 co-receptor.[59],[62] The mutation within the CCR-2 gene is a conservative valine to isoleucine at position 64 in the first transmembrane domain of the CCR-2 receptor, which is present at an allelic frequency of 10-25% in different populations.[59] The presence of this mutation in either heterozygote or homozygote has been associated with delayed progression to AIDS and death in most, although not all, cohorts. In contrast with CCR-5-D-32 mutation, it provides protection against HIV disease progression in races other than the whites.[57],[68],[69],[70],[71] Increased frequency of CCR-2-64I homozygosity was observed in exposed uninfected individuals compared with HIV positive and HIV negative controls suggesting an association between CCR-2 64I homozygosity and resistance to heterosexual transmission.[18] However the mechanism of protection is not clear, since the CCR-2 is only rarely used as a co-receptor by HIV. It has been suggested that CCR-2 64I mutation tracks with another mutation through linkage disequilibrium, particularly in the regulatory or promoter region of CCR-5.[58],[66],[68] A polymorphism within the regulatory region of CCR-5 59653T is in linkage disequilibrium with the 64I mutation, but the functional significance of this finding is unclear.[68]

SDF-1 3' αmutation

Another genetic trait that might affect progression to AIDS involves stromal cell derived factor-1 (SDF-1), the chief ligand of CXCR4. SDF-1 blocks infection with X-4 variant of HIV-1.[72],[73] The mutation SDF-1 3'α involves a G/A transition at position 801 of SDF-1 3' α untranslated region (UTR) and is associated with protection against HIV.[74] This mutation is common among all geographical regions of the world. Mutation may upregulate the synthesis of SDF-1, thus competitively inhibiting X 4 HIV from binding. Persons who are homozygous for this mutation have been shown to experience delayed progression to AIDS but do not exhibit decreased susceptibility to infection with HIV.[58],[74],[75] In contrast, other studies have shown that SDF-1 3' α homozygosity was associated with accelerated disease progression[66],[76],[77] and increased viral replication[78] or no effect on disease progression.[60]

Other mutations

Two single nucleotide polymorphism (SNP) sites, a cytosine to guanine transversion polymorphism at position - 28 (RANTES 28 C/G) and a guanine to adenine transition polymorphism at position - 403 (RANTES 403 G/A), in the promoter region of RANTES have been identified. RANTES 403 A and 28 G promoter polymorphism is associated with increased RANTES transcription and delayed progression to AIDS in HIV infected individuals.[79],[80] No mutation has been described in the CXCR-4 region that might influence HIV infection.

Anti HIV-1 therapy based on chemokines and their receptors

The discovery of chemokine receptors as co-receptors for HIV-1 has opened the door for a number of novel antiviral approaches. The need for an improvement of current drug combination regimens, based on inhibitors of the viral reverse transcriptase and protease, is underscored by a series of important drawbacks, including the rapid rebound after withdrawal, the increasing emergence and transmission of multidrug resistance, side effects, difficulties in schedule compliance and, most importantly, the lack of virus eradication even after long term effective treatment.[81] While all the currently licensed antiviral drugs except peptide T 20 block HIV after its penetration into the target cell, co-receptor inhibitors target the early interactions between virus and cell membrane, thereby blocking HIV outside its target cells. However, major hurdles in the way towards developing safe and effective co-receptor inhibitors is the risk of interfering with the physiology of the chemokine system, causing potentially harmful side effects.[81] Therapeutic agents that interfere with chemokine co-receptors might also block membrane fusion and viral entry. Agents that block or prevent CCR-5 might prove useful and safe in the prevention of and treatment for HIV infection. The known inhibitory effects of CCR-5 ligands, RANTES, MIP-1a and MIP-1b, have led to the consideration of their use as potential therapeutic agents to limit HIV entry. But this approach has its own disadvantages as this might recruit HIV susceptible cells through chemotaxis, increase X 4 virus replication and even increase infectivity of R 5 viruses.[82],[83]

Chemokine receptor antagonists

Another therapeutic approach is the usage of chemokine receptor antagonists. In this regard, a number of studies have demonstrated that N-terminal modification and truncation of chemokines can give rise to specific receptor antagonists. This approach has been used to create potential chemokine antagonists of HIV-1 co-receptors. The advantage of this approach is that these molecules can block HIV without activating chemotaxis or proinflammatory effect and without increasing the level of X 4 HIV.

CCR-5 inhibitors

Various CCR-5 ligands with antiviral properties have been described, including modified chemokines and monoclonal antibodies and more importantly small-molecule inhibitors with potential for oral administration Example for such modified or truncated CCR-5 antagonists are Aminooxypentane (AOP), N-nonanoyl (NNY)-RANTES, N (n-nonanoyl)-des-Ser (PSC) RANTES 9-68 RANTES and met RANTES. These compounds induce CCR-5 internalization.[84],[85],[86],[87] Another study has generated a panel of recombinant RANTES analogues bearing natural amino acid substitution at the amino-terminus and two of them, L-RANTES and C1.C5-RANTES have anti HIV potency.[81] Another study reported a series of 1, 3, 4-trisubstituted pyrrolidine CCR-5 receptor antagonists containing a variety of fused heterocycles at the 4-position of the piperidine side chain with potent anti-HIV activity[89] A number of other small molecular weight compound like Tak 779,[90] Tak 220,[91] the spirodiketopeperazine derivative E-913,[92] monoclonal antibody PRO-140,[93] the small molecular weight compounds SHC-C and SHC-D[94] are under advanced clinical trials . Antiviral activity of anti CCR 5 monoclonal antibody PRO 140 was investigated.[93] In this study, PRO 140 is tested against a panel of primary HIV-1 isolates and the result showed that, low nanomolar concentrations of PRO 140 inhibited infection of primary peripheral blood mononuclear cells (PBMC) by all CCR-5 using (R 5) viruses tested.[93]

CXCR-4 inhibitors

In addition to modified chemokines, a peptide inhibitor of CXCR4 known as T 22 was identified. It is able to inhibit specifically the ability of T cell tropic HIV-1 which uses CXCR- 4 co-receptor but not R5 HIV-1. In addition, T 22 also inhibits Ca 2+ mobilization induced by SDF-1 stimulation through CXCR-4. Another report, describes an inhibitor of CXCR-4 known as Allelix (ALX)-40-4C. This compound is a highly cationic oligopeptide containing nine arginine residues. ALX 40-4C inhibits CXCR-4 dependent HIV-1 mediated membrane fusion and viral entry by T and dual tropic HIV-1 strains, but does not inhibit HIV-1 fusion mediated by CCR-5. In addition, ALX- 40-4C blocks SDF-1 mediated activation of CXCR-4 and binding of the CXCR-4 specific monoclonal antibodies12 G 5 to cells expressing CXCR-4.[95],[96] Several other modified ligands of CXCR-4 receptor that are under investigation include peptoids CGP-64222, arginine conjugate such as R3G and NeoR, the bycyclams and the recently reported AMD070 and KRH 1636.[97],[98],[99],[100],[101],[102],[103],[104] The bycyclam AMD-3100 blocks HIV-1 entry through CXCR-4 and inhibits binding of SDF-1 and 12G5 to CXCR-4 but does not itself trigger cell signaling.[101] AMD-3100 does not inhibit the binding of CC chemokine ligands to CCR-1, CCR-2b or CCR-5.

Another strategy to prevent HIV-1 infection is to reduce the surface expression of level of HIV-1 co-receptors. This principle has been used by some studies to develop a device to trap the HIV-1 co-receptors CCR-5 and CXCR-4 in the endoplasmic reticulum (ER), thus preventing their transport to cell surface.[105] Other areas of investigations are the administration of CD-4 cells with decreased CCR-5 expression, gene therapy to prevent receptor expression through antibodies or altered ligands and development of pseudo viruses or vectors that express CD-4 and chemokine receptors and thus could target HIV infected cells to deliver antiviral treatment or kill HIV infected cells. "Short interfering RNA" (siRNA) represent a new molecular tool that is able to selectively inactivate target genes.[106] Double stranded RNA is split by the enzyme dicer-1 into short pieces. This oligomer may complimentarily bind to longer RNA sequences that are subsequently degraded. The use of siRNA against CCR-5 can prevent the expression of CCR-5 in vitro .[106],[107],[108] Another approach based on a hammerhead ribozyme and an RNA cleaving DNA enzyme was used against CCR-5 and shown that when these ribozyme under a strong eukaryotic promoter are introduced in to a mammalian cell, could interfere the co-receptor function.[108] The in vivo effectiveness of these molecular tools needs to be evaluated extensively before concluding its role in interfering co-receptor functions.

Chemokine as topical microbicides

Approaches using chemokine analog as a topical microbicide at the site of viral entry have been investigated in animal models. A study reported that topical application of high doses of PSC-RANTES, an amino terminus-modified analog of the chemokine RANTES, provided potent protection against intravaginal challenge in rhesus macaques without detectable toxicity or histological changes.[109],[110]

Few studies using CMPD167, a small molecule that binds to CCR-5 to inhibit gp 120 association, were found to be protective against vaginal transmission of R 5 virus in rhesus macaque. These findings support the development of small molecule CCR-5 inhibitors as antiviral therapies and possibly as components of a topical microbicide to prevent HIV-1 sexual transmission.[111],[112]

Issues associated with the usage of chemokine and its antagonists in HIV therapy

Blocking cellular receptors also presents challenges due to potential toxic effects from interference with their normal function in lymphocyte development and trafficking and inflammation. The following mechanisms of escape from co-receptor inhibitors are possible: (1) the escape mutant may continue to use the same co-receptor in an inhibitor-insensitive manner; (2) co-receptor switching may occur, so that R 5 viruses become able to use CXCR-4, or vice versa . CXCR-4 receptor and its ligand SDF-1 are involved in various physiologic processes like B cell lymphopoesis, cardiac and cerebellar development in embryogenesis, bone marrow myelopoisis and vascularization of the gastrointestinal tract.[113],[114],[115] Therefore, the clinical application of CXCR-4 blockers may be limited. A further challenge for agents targeting viral entry is the ability of HIV to become resistant to the selective pressure exerted by the drug. Even when given in the context of other antiviral agents, a low level of viral replication may suffice to select viral variants that use alternative co-receptors or entry mechanisms. This possibility of viral evolution has to be considered before using receptor antagonists in HIV therapy.[116] It is reported that acquisition of CXCR-4 use is not the dominant in vitro escape pathway for a small molecule, CCR-5 entry inhibitor. Instead, HIV-1 acquires the ability to use CCR-5 despite the inhibitor, first by requiring lower levels of CCR-5 for entry and then probably by using the drug-bound form of the receptor.[117] Another study has suggested that co-receptor expression levels also influenced sensitivity to fusion inhibitors and fusion kinetics. Thus, receptor expression levels and env /receptor affinity are cellular and viral determinants, respectively, that impact viral sensitivity to different classes of entry inhibitors. Therefore, mutations that result in drug resistance may do so directly by altering inhibitor binding sites or indirectly by affecting the rate of membrane fusion. Individuals who express lower levels of CCR-5, such as CCR-5-32 heterozygotes, may consequently respond more favorably entry inhibitors and viruses that exhibit enhanced affinity for co-receptor may respond less well.[118] Moreover, entry inhibitors must overcome the obstacles such as their pharmacological toxic effects, bioavailability and affordability. In gene therapy, introduction of pseudoviruses or vectors into immunocompromised individuals might result in vector associated morbidity and mortality as these individuals might not be able to contain the replication and dissemination of vectors.

 :: Conclusion Top

Research is progressing rapidly in the area of chemokines and their receptors in HIV pathogenesis. The coming together of the two major fields of research, HIV and chemokines, will produce significant new insights and new therapies that will enable us to combat HIV infection. Since the identification of the chemokine receptors as co-receptors for HIV entry, many of the mysteries of HIV pathogenesis have become clear. However, the mechanisms that allow interaction of HIV-1 with chemokine receptors and implications of virus -induced receptor signaling are yet to be answered. Moreover, the potential of this discovery for anti-HIV therapy and vaccine development remains to be explored further. The other areas like role of various chemokines in HIV pathogenesis in vivo and the mechanism of genetic host factors mediated protection need to be investigated further.

 :: References Top

1.Walker CM, Moody DJ, Stites DP, Levy JA. CD8 + lymphocytes can control HIV infection in vitro by suppressing virus replication. Science 1986;234:1563-6.  Back to cited text no. 1  [PUBMED]  
2.Levy JA, Mackewicz CE, Barker E. Controlling HIV pathogenesis: The role of the noncytotoxic anti-HIV response of CD8+ T Cells. Immunol Today 1996;17:217-24.   Back to cited text no. 2    
3.Cocchi F, de Vico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P. Identification of RANTES, MIP- 1 alpha and MIP-1 beta as the major HIV suppressive factors produced by CD8+ T cells. Science 1995;270:1811-5.  Back to cited text no. 3    
4.Berger EA, Murphy PM, Farber JM. Chemokine receptors as HIV-1 co-receptors: Roles in viral entry, tropism and disease. Annu Rev Immunol 1999;17:657-700.  Back to cited text no. 4    
5.Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: Functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 1996;272:872-7.  Back to cited text no. 5    
6.Deng H, Liu R, Ellmeier W, Choe S, Unutmaz D, Burkhart M, et al . Identification of a major co-receptor for primary isolates of HIV-1. Nature 1996;381:661-6.  Back to cited text no. 6    
7.Dragic T, Litwin V, Allaway GP, Martin SR, Huang Y, Nagashima KA, et al . HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature 1996;381:667-73.  Back to cited text no. 7    
8.Alkhatib G, Combadiere C, Broder CC, Feng Y, Kennedy PE, Murphy PM, Berger EA. CC-CKR5: A RANTES, MIP- 1 alpha, MIP- 1 beta receptor as fusion cofactor for macrophage-tropic HIV-1. Science 1996;272:1955-8.  Back to cited text no. 8    
9.Choe H, Farzan M, Sun Y, Sullivan Y, Rollins B, Ponath PD, et al . The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell 1996;85:1135-48.  Back to cited text no. 9    
10.Doranz BJ, Rucker J, Yi Y, Smyth RJ, Samson M, Peiper SC, et al . A dual-tropic primary HIV-1 isolate that uses fusin and the beta chemokine receptors CKR-5, CKR-3 and CKR-2b as fusion cofactors. Cell 1996;85:1149-58.  Back to cited text no. 10    
11.Rizzuto CD, Wyatt R, Hernandez-Ramos N, Sun Y, Kwong PD, Hendrickson WA, et al . A conserved HIV gp120 envelope glycoprotein structure involved in chemokine receptor binding. Science 1998;280:1949-53.  Back to cited text no. 11    
12.Wyatt R, Sodroski J. The HIV-1 envelope glycoproteins: Fusogen, antigens and immunogens. Science 1998;280:1884-8.  Back to cited text no. 12    
13.Wu L, Gerard NP, Wyatt R, Choe H, Parolin C, Ruffing N, et al . CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR5. Nature 1996;384:179-83.  Back to cited text no. 13    
14.Berger EA, Doms RW, Fenyo EM, Korber BT, Littman DR, Moore JP, et al . A new classification for HIV-1. Nature 1998;391:240.  Back to cited text no. 14    
15.Simmons G, Wilkinson D, Reeves JD, Dittmar MT, Beddows S, Weber J, et al . Primary, syncytium-inducing human immunodeficiency virus type 1 isolates are dual-tropic and most can use either Lestr or CCR5 as co-receptors for virus entry. J Virol 1996;70:8355-60.  Back to cited text no. 15    
16.Deng HK, Unutmaz D, Kewal Ramani VN, Littman DR. Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 1997;388:296-300.  Back to cited text no. 16    
17.Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL-33, a novel chemokine receptor-like protein, functions as fusion cofactor for both macrophage-tropic and T cell tropic HIV-1. J Exp Med 1997;185:2015-23.  Back to cited text no. 17    
18.Louisirirotchanakul S, Liu H, Roongpisuthlpong A, Nakayama EE, Takebe Y, Shioda T, et al . Genetic analysis of HIV-1 discordant couples in Thailand: Association of CCR2 64I homozygosity with HIV-1 negative status. J Acquir Immune Defic Syndr 2002;29:314-5.  Back to cited text no. 18    
19.Neil SJ, Aasa-Chapman MM, Clapham PR, Nibbs RJ, McKnight A, Weiss RA. The promiscuous CC chemokine receptor D6 is a functional co-receptor for primary isolates of human immunodeficiency virus type 1 (HIV-1) and HIV-2 on astrocytes. J Virol 2005;79:9618-24.  Back to cited text no. 19    
20.Luster AD. Chemokines- chemotactic cytokines that mediate inflammation. N Eng J Med 1998;338:436-45.  Back to cited text no. 20    
21.Hernanz-Falcon P, Rodriguez-Frade JM, Serrano A, Juan D, delSol A, Soriano SF, et al . Identification of amino acid residues crucial for chemokine receptor dimerization. Nat Immunol 2004;5:216-23.  Back to cited text no. 21    
22.Rodriguez-Frade JM, del Real G, Serrano A, Hernanz-Falcon P, Soriano SF, Vila-Coro AJ, et al . Blocking of HIV infection via CCR5 and CXCR-4 receptors by acting in trans on the CCR2 chemokine receptor. EMBO J 2004;23:66-76.  Back to cited text no. 22    
23.Vila-Coro AJ, Rodriguez-Frade JM, Martin De Ana A, Moreno-Ortiz MC, Martinez-AC, Mellado M. The chemokine SDF-1alpha triggers CXCR4 receptor dimerization and activates the JAK/STAT pathway. FASEB J 1999;13:1699-71.  Back to cited text no. 23    
24.Garzino-Demo A, Moss RB, Margolick JB, Cleghorn F, Still A, Blattner WA, et al . Spontaneous and antigen induced production of HIV inhibitory b-chemokines are associated with AIDS free status. Proc Natl Acad Sci USA 1999;96:11986-91.  Back to cited text no. 24    
25.Ullum H, Cozzi Lepri A, Victor J, Aladdin H, Phillips AN, Gerstoft J, et al . Production of beta-chemokines in human immunodeficiency virus (HIV) infection: Evidence that high levels of macrophage inflammatory protein-1 beta are associated with a decreased risk of HIV disease progression. J Infect Dis 1998;177:331-6.  Back to cited text no. 25    
26.Furci L, Scarlatti G, Burastero S, Tambussi G, Colognesi C, Qulillent C, et al . Antigen-driven C-C chemokine-mediated HIV-1 suppression by CD4+ T cells from exposed uninfected individuals expressing the wild-type CCR5 allele. J Exp Med 1997;186:455-60.  Back to cited text no. 26    
27.Shieh B, Yan YP, Ko NY, Liau YE, Liu YC, Lin HH, et al . Detection of elevated serum beta-chemokine levels in seronegative Chinese individuals exposed to human immunodeficiency virus type 1. Clin Infect Dis 2001;33:273-9.  Back to cited text no. 27    
28.Skurnick JH, Palumbo P, de Vico A, Shacklett BL, Valentine FT, Merges M, et al . Correlates of non transmission in US women at high risk of human immunodeficiency virus type 1 infection through sexual exposure. J Infect Dis 2002;185:428-38.  Back to cited text no. 28    
29.Saha K, Bentsman G, Chess L, Volsky DJ. Endogenous production of beta-chemokines by CD4+, but not CD8+, T-cell clones correlates with the clinical state of human immunodeficiency virus type-1 (HIV-1) individuals and may be responsible for blocking infection with non-syncytium-inducing HIV-1 in vitro. J Virol 1998;72:876-81.  Back to cited text no. 29    
30.Cocchi F, de Vico AL, Yarchoan R, Redfield R, Cleghorn F, Blattner WA, et al . Higher macrophage inflammatory protein (MIP) 1-alpha and MIP1-beta levels from CD8+ T cells are associated with asymptomatic HIV-1 infection. Proc Natl Acad Sci USA 2000;97:13812-7.   Back to cited text no. 30    
31.Mazzoli S, Trabattoni D, Lo Caputo S, Piconi S, Ble C, Meacci F, et al . HIV-specific mucosal and cellular immunity in HIV-seronegative partners of HIV-seropositive individuals. Nat Med 1997;3:1250-7.  Back to cited text no. 31    
32.Paxton WA, Liu R, Kang S, Wu L, Gingeras TR, Landau NR, et al . Reduced HIV-1 infectability of CD4+ lymphocytes from exposed uninfected individuals: Association with low expression of CCR5 and high production of beta-chemokines. Virology 1998;244:66-73.  Back to cited text no. 32    
33.Fowke KR, Dong T, Rowland-Jones SL, Oyugi J, Rutherford WJ, Kimani J, et al . HIV type 1 resistance in Kenyan sex workers is not associated with altered cellular susceptibility to HIV type 1 infection or enhanced beta-chemokine production. AIDS Res Hum Retroviruses 1998;14:1521-30.  Back to cited text no. 33    
34.Sriwanthana B, Hodge T, Mastro TD, Dezzutti CS, Bond K, Stephens HA, et al . HIV specific cytotoxic T lymphocytes, HLA A11 and chemokine-related factors may act synergistically to determine HIV resistance in CCR5 delta 32 negative female sex workers in Chiang Rai, Northern Thailand. AIDS Res Hum Retrov 2001;17:719-34.  Back to cited text no. 34    
35.Liu H, Chao D, Nakayama EE, Taguchi H, Goto M, Xin X, et al . Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. Proc Natl Acad Sci USA 1999;96:4581-5.  Back to cited text no. 35    
36.Belec L, Ghys PD, Hocini H, Nkengasong JN, Tranchot-Diallo J, Diallo MO, et al . Cervicovaginal secretory antibodies to human immunodeficiency virus type 1 (HIV-1) that block viral transcytosis through tight epithelial barriers in highly exposed HIV-1 seronegative African women. J Infect Dis 2001;184:1412-22.  Back to cited text no. 36    
37.Butera ST, Pisell TL, Limpakarnjanarat K, Young NL, Hodge TW, Mastro TD, et al . Production of a novel viral suppressive activity associated with resistance to infection among female sex workers exposed to HIV type 1. AIDS Res Hum Retroviruses 2001;17:735-44.  Back to cited text no. 37    
38.Schmidtmayerova H, Sherry B, Bukrinsky M. Chemokines and HIV replication [Letter]. Nature 1996;382:767.  Back to cited text no. 38    
39.Schmidtmayerova H, Nottet HS, Nuovo G, Raabe T, Flanagan CR, Dubrovsky L, et al . Human immunodeficiency virus type 1 infection alters chemokine beta peptide expression in human monocytes: Implications for recruitment of leukocytes into brain and lymph node. Proc Natl Acad Sci USA 1996;93:700-4.  Back to cited text no. 39    
40.Canque B, Rosenzwajg M, Gey A, Tartour E, Fridman WH, Gluckman JC, et al . Macrophage inflammatory protein-1alpha is induced by human immunodeficiency virus infection of monocyte-derived macrophages. Blood 1996;87:2011-9.  Back to cited text no. 40    
41.Lane BR, King SR, Bock PJ, Strieter RM, Coffey MJ, Markovitz DM. The C-X-C chemokine IP-10 stimulate HIV-1 replication. Virology 2003;307:122-34.  Back to cited text no. 41    
42.Lu B, Rutledge BJ, Gu L, Fiorillo J, Lukacs NW, Kunkel SL, et al . Abnormalities in monocyte recruitment and cytokine expression in monocyte chemoattractant protein 1-deficient mice. J Exp Med 1998;187:601-8.  Back to cited text no. 42    
43.Conant K, Garzino-Demo A, Nath A, McArthur JC, Halliday W, Power C, et al . Induction of monocyte chemoattractant protein-1 in HIV-1 Tat-stimulated astrocytes and elevation in AIDS dementia. Proc Natl Acad Sci USA 1998;95:3117-21.  Back to cited text no. 43    
44.Narimatsu R, Wolday D, Patterson BK. IL-8 increases transmission of HIV type 1 in cervical explant tissue. AIDS Res Hum Retroviruses 2005;21:228-33.  Back to cited text no. 44    
45.Izmailova E, Bertley FM, Huang Q, Makori N, Miller CJ, Young RA, et al . HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages. Nat Med 2003;9:191-7.  Back to cited text no. 45    
46.Reinhart TA. Chemokine induction by HIV-1: Recruitment to the cause. Trends Immunol 2003;24:351-3.   Back to cited text no. 46    
47.Liu R, Paxton WA, Choe S, Ceradini D, Martin SR, Horuk R, et al . Homozygous defect in HIV-1 co-receptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell 1996;86:367-77.  Back to cited text no. 47    
48.Dean M, Carrington M, Winkler C, Huttley GA, Smith MW, Allikmets R, et al . Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CKR5 structural gene. Hemophilia Growth and Development Study, Multicenter AIDS Cohort Study, Multicenter Hemophilia Cohort Study, San Francisco City cohort, ALIVE Study. Science 1996;273:1856-62.  Back to cited text no. 48    
49.Biti R, Ffrench R, Young J, Bennetts B, Stewart G, Liang T. HIV-1 infection in an individual homozygous for the CCR5 deletion allele. Nat Med 1997;3:252-3.  Back to cited text no. 49    
50.Huang Y, Paxton WA, Wolinsky SM, Neumann AU, Zhang L, He T, et al . The role of mutant CCR5 allele in HIV-1 transmission and disease progression. Nat Med 1996;2:1240-3.  Back to cited text no. 50    
51.Zimmerman PA, Buckler-White A, Alkhatib G, Spalding T, Kubofcik J, Comdadiere C, et al . Inherited resistance to HIV-1 conferred by an inactivating mutation in CC chemokine receptor 5: Studies in populations with contrasting clinical phenotypes, defined racial background and quantified risk. Mol Med 1997;3:23-36.  Back to cited text no. 51    
52.Balotta C, Bagnarelli P, Violin M, Ridolfo AL, Zhou D, Berlusconi A, et al . Homozygous delta 32 deletion of the CCR5 chemokine receptor gene in an HIV-1 infected patient. AIDS 1997;11:F67-71.  Back to cited text no. 52    
53.Theodorou I, Meyer L, Magierowska M, Katlama C, Rouzioux C. HIV-1 infection in an individual homozygous for CCR5 delta 32. Seroco Study Group [Letter]. Lancet 1997;349:1219-20.  Back to cited text no. 53    
54.Gray L, Churchill MJ, Keane N, Sterjovski J, Ellett AM, Purcell DF, et al . Genetic and functional analysis of R5X4 human immunodeficiency virus type 1 envelope glycoproteins derived from two individuals homozygous for the CCR5delta32 allele. J Virol. 2006;80:3684-91.  Back to cited text no. 54    
55.Gorry PR, Zhang C, Wu S, Kunstman K, Trachtenberg E, Phair J, et al . Persistence of dual-tropic HIV-1 in an individual homozygous for the CCR5 Delta 32 allele. Lancet 2002;359;1832-4.  Back to cited text no. 55    
56.Paxton WA, Martin SR, Tse D, O'Brien TR, Skurnick J, VanDevanter NL, et al . Relative resistance to HIV-1 infection of CD4 lymphocytes from person who remain uninfected despite multiple high-risk sexual exposure. Nat Med 1996;2:412-7.  Back to cited text no. 56    
57.Smith MW, Dean M, Carrington M, Winkler C, Huttley GA, Lomb DA, et al . Contrasting genetic influence of CCR2 and CCR5 variants on HIV-1 infection and disease progression. Hemophilia Growth and Development Study (HGDS), Multicenter AIDS Cohort Study (MACS), Multicenter Hemophilia Cohort Study (MHCS), San Francisco City cohort (SFCC), ALIVE Study. Science 1997;277:959-65.  Back to cited text no. 57    
58.Martin MP, Dean M, Smith MW, Winkler C, Gerrard B, Michael NL, et al . Genetic acceleration of AIDS progression by a promoter variant of CCR5. Science 1998;282:1907-11.  Back to cited text no. 58 Roda Husman AM, Koot M, Cornelissen M, Keet IP, Brouwer M, Brorsen SM, et al . Association between CCR5 genotype and the clinical course of HIV-1 infection. Ann Intern Med 1997;127:882-90.  Back to cited text no. 59    
60.Meyer L, Magierowska M, Hubert JB, Theodorou I, van Rij R, Prins M, et al . CC-chemokine receptor variants, SDF-1 polymorphism and disease progression in 720 HIV-infected patients. SEROCO Cohort. Amsterdam Cohort Studies on AIDS [Letter]. AIDS 1999;13:624-6.  Back to cited text no. 60    
61.Ioannidis JP, O'Brien TR, Rosenberg PS, Contopoulos-Ioannidis DG, Goedert JJ. Genetic effects on HIV disease progression [Letter]. Nat Med 1998;4:536.  Back to cited text no. 61    
62.Carrington M, Kissner T, Gerrard B, Ivanov S, O'brien SJ, Dean M. Novel alleles of the chemokine-receptor gene CCR5. Am J Hum Genet 1997;61:1261-7.  Back to cited text no. 62    
63.Quillent C, Oberlin E, Braun J, Rousset D, Gonzalez-Canali G, Metais P, et al . HIV-1-resistance phenotype conferred by combination of two separate inherited mutations of CCR5 gene. Lancet 1998;351:14-8.  Back to cited text no. 63    
64.Mummidi S, Ahuja SS, McDaniel BL, Ahuja SK. The human CC chemokine receptor 5 (CCR5) gene. Multiple transcripts with 5'-end heterogeneity, dual promoter usage and evidence for polymorphisms within the regulatory regions and noncoding exons. J Biol Chem 1997;272:30662-71.  Back to cited text no. 64    
65.McDermott DH, Zimmerman PA, Guignard F, Kleeberger CA, Leitman SF, Murphy PM. CCR5 promoter polymorphism and HIV-1 disease progression. Muticenter AIDS Cohort Study (MACS). Lancet 1998;352:866-70.  Back to cited text no. 65    
66.Mummidi S, Ahuja SS, Gonzalez E, Anderson SA, Santiago EN, Stephan KT, et al . Genealogy of the CCR5 locus and the chemokine system gene variants associated with altered rates of HIV-1 progression. Nat Med 1998;4:786-93.  Back to cited text no. 66    
67.Kostrikis LG, Neumann AU, Thomson B, Korber BT, McHardy P, Karanicolas R, et al . A polymorphism in the regulatory region of the CC-chemokine receptor 5 gene influences perinatal transmission of human immunodeficiency virus type-1 to African-American infants. J Virol 1999;73:10264-71.  Back to cited text no. 67    
68.Kostrikis LG, Huang Y, Moore JP, Wolinsky SM, Zhang L, Guo Y, et al . A chemokine receptor CCR2 allele delays HIV-1 disease progression and is associated with a CCR5 promoter mutation. Nat Med 1998;4:350-3.  Back to cited text no. 68    
69.Anzala AO, Ball TB, Rostron T, O'Brien SJ, Plummer FA, Rowland-Jones SL. CCR2-64I allele and genotype association with delayed AIDS progression in African women. University of Nairobi Collaboration for HIV Research. Lancet 1998;351:1632-3.  Back to cited text no. 69    
70.Mulherin SA, O'Brien TR, Ioannidis JP, Goedert JJ, Buchbinder SP, Coutinho RA, et al . Effects of CCR5-Delta32 and CCR2-64I alleles on HIV-1 disease progression: The protection varies with duration of infection. AIDS 2003;17:377-87.  Back to cited text no. 70    
71.Mazzucchelli R, Corvasce S, Violin M, Riva C, Bianchi R, Deho L, et al . Role of CCR5, CCR2 and SDF-1 gene polymorphisms in a population of HIV-1 infected individuals. J Biol Regul Homeost Agents 2001;15:265-71.  Back to cited text no. 71    
72.Bleul CC, Farzan M, Choe H, Parolin C, Clark-Levis I, Sodrosky J, et al . The lymphocyte chemoattractant SDF-1 is the ligand for LESTR/fusin and blocks HIV-1 entry. Nature 1996;382:829-33.  Back to cited text no. 72    
73.Oberlin E, Amara A, Bachelerie F, Bessia C, Virelizier JL, Arenzana-Seisdedos F, et al . The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature 1996;382:833-5.  Back to cited text no. 73    
74.Winkler C, Modi W, Smith M, Nelson GW, Wu X, Carrington M, et al . Genetic restriction of AIDS pathogenesis by an SDF-1 chemokine gene variant. ALIVE Study, Hemophilia Growth and Development Study (HGDS), Multicenter AIDS Cohort Study (MACS), Multicenter Hemophilia Cohort Study (MHCS), San Francisco City Cohort (SFCC). Science 1998;279:389-93.  Back to cited text no. 74    
75.Hendel H, Henon N, Lebuanec H, Lachgar A, Poncelet H, Caillat-Zucman S, et al . Distinctive effect of CCR5, CCR2 and SDF1 genetic polymorphism in AIDS progression. J Acquir Immune Defic Syndr Hum Retrovirol 1998;19:381-6.  Back to cited text no. 75    
76.van Rij RP, Broersen S, Goudsmit J, Coutinho RA, Schuitemaker H. The role of a stromal cell-derived factor-1 chemokine gene variant in the clinical course of HIV-1 infection. AIDS 1998;12:F85-90.  Back to cited text no. 76    
77.Brambilla A, Villa C, Rizardi G, Veglia F, Ghezzi S, Lazzarin A, et al . Shorter survival of SDF-1-3'A/3'A homozygotes linked to CD4+ T cell decrease in advanced human immunodeficiency virus type-1 infection. J Infect Dis 2000;182:311-5.  Back to cited text no. 77    
78.Balotta C, Bagnarelli P, Corvasce S, Mazzucchelli R, Colombo MC, Papagno L, et al . Identification of two distinct subsets of long term nonprogressors with divergent viral activity by stromal-derived factor 1 chemokine gene polymorphism analysis. J Infect Dis 1999;180:285-9.  Back to cited text no. 78    
79.McDermott DH, Beecroft MJ, Kleeberger CA, Al-Sharif FM, Ollier WE, Zimmerman PA, et al . Chemokine RANTES promoter polymorphism affects risk of both HIV infection and disease progression in the Multiceter AIDS Cohort Study. AIDS 2000;14:2671-8.  Back to cited text no. 79    
80.Liu H, Chao D, Nakayama EE, Taguchi H, Goto M, Xin X, et al . Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. Proc Natl Acad Sci USA 1999;96:4581-5.  Back to cited text no. 80    
81.Lusso P. HIV and chemokines: Implications for therapy and vaccine. Vaccine 2002;20:1964-7.  Back to cited text no. 81    
82.Trkola A, Paxton WA, Monard SP, Hoxie JA, Siani MA, Thompson DA, et al . Genetic subtype-independent inhibition of human immunodeficiency virus type 1 replication by CC and CXC chemokines. J Virol 1998;72:396-404.  Back to cited text no. 82    
83.Gordon CJ, Muesing MA, Proudfoot AE, Power CA, Moore JP, Trkola A. Enhancement of human immunodeficiency virus type 1 infection by the CC-chemokine RANTES is independent of the mechanism of virus-cell fusion. J Virol 1999;73:684-94.  Back to cited text no. 83    
84.Mack M, Luckow B, Nelson PJ, Cihak J, Simmons G, Clapham PR, et al . Aminooxypentane-RANTES induces CCR5 internalization but inhibit recycling: A novel inhibitory mechanism of HIV infectivity. J Exp Med 1998;187:1215-24.  Back to cited text no. 84    
85.Simmons G, Clapham PR, Picard L, Offord RE, Rosenkilde MM, Schwartz TW, et al . Potent inhibition of HIV-1 infectivity in macrophages and lymphocytes by a novel antagonist. Science 1997;276:276-9.  Back to cited text no. 85    
86.Arenzana-Seisdedos F, Virelizier JL, Rousset D, Clark-Lewis I, Loetscher P, Moser B, et al . HIV blocked by chemokine antagonist. Nature 1996;383:400.  Back to cited text no. 86    
87.Ylisastigui L, Vizzavona J, Drakopoulou E, Paindavoine P, Calvo CF, Parmentier M, et al . Synthetic full-length and truncated RANTES inhibit HIV-1 infection of primary macrophages. AIDS 1998;12:977-84.  Back to cited text no. 87    
88.Proudfoot A, Power CA, Hoogewerf AJ, Montjovent MO, Borlat F, Offord RE, et al . Extention of recombinant human RANTES by the retention of the initiating methionine produces a potent antagonist. J Biol Chem 1996;271:2599-603.  Back to cited text no. 88    
89.Kim D, Wang L, Hale JJ, Lynch CL, Budhu RJ, Maccoss, et al . Potent 1,3,4-trisubstituted pyrrolidine CCR5 receptor antagonists: Effects of fused heterocycles on antiviral activity and pharmacokinetic properties. Bioorg Med Chem Lett 2005;15:2129-34.  Back to cited text no. 89    
90.Baba M, Nishimura O, Kanzaki N, Okamoto M, Sawada H, Iizawa Y, et al . A small-molecule, non peptide CCR5 antagonist with highly potent and selective anti HIV-1 activity. Proc Natl Acad Sci USA 1999;96:5698-703.  Back to cited text no. 90    
91.Este JA. Virus entry as a target for anti HIV intervention. Curr Med Chem 2003;10:1617-32.  Back to cited text no. 91    
92.Maeda K, Yoshimura K, Shibayama S, Habashita H, Tada H, Sagawa K, et al . Novel low molecular weight spirodiketopeperazine derivative potently inhibit R5 HIV-1 inhibition through their antagonistic effect on CCR5. J Biol chem 2001;276:35194-200.  Back to cited text no. 92    
93.Trkola A, Ketas TJ, Nagashima KA, Zhao L, Cilliers T, Morris L, et al . Potent, broad spectrum inhibition of human immunodeficiency virus type-1 by the CCR5 monoclonal antibody PRO 140. J Virol 2001;75:579-88.  Back to cited text no. 93    
94.Baroudy BM. Second generation CCR5 antagonist that inhibits HIV-1 entry. Program and abstracts of The 1st IAS Conference on HIV Pathogenesis and Treatment. Buenos Aires: Argentina; 2001, Abstract 70.  Back to cited text no. 94    
95.Murakami T, Nakajima T, Koyanagi Y, Ttachibana K, Fujii N, Tamamura H, et al . A small molecule CXCR4 inhibitor that blocks T cell line tropic HIV-1 infection. J Exp Med 1997;186:1389-93.  Back to cited text no. 95    
96.Doranz BJ, Grovit-Ferbas K, Sharron MP, Mao SH, Goetz MB, Daar ES, et al . A small molecule inhibitor directed against the chemokine receptor CXCR4 prevents its use as an HIV-1 co-receptor. J Exp Med 1997;186:1395-400.  Back to cited text no. 96    
97.Cabrera C, Gutierrez A, Barretina J, Blanco J, Litovchick A, Lapidot A, et al . Anti HIV activity of novel aminoglycoside-arginine conjugate. Antiviral Res 2002;53:1-8.  Back to cited text no. 97    
98.Cabrera C, Gutierrez A, Blanco J, Barrentina J, Litovchick A, Lapidot A, et al . Anti-human immunodeficiency virus activity of novel aminoglycoside-arginine conjugates at early stages of infection. AIDS Res Hum Retroviruses 2000;16:627-34.  Back to cited text no. 98    
99.Daelemans D, Schols D, Witvrouw M, Pannecouque C, Hatse S, van Dooren S, et al . A second target for the peptoid Tat/transactivation response element inhibitor CGP64222: Inhibition of human immunodeficiency virus replication by blocking CXC-chemokine receptor 4 mediated virus entry. Mol Pharmacol 2000;57:116-24.  Back to cited text no. 99    
100.Este JA, Cabrera C, de Clercq E, Struyf S, Van Damme J, Bridger G, et al . Activity of different bicyclam derivatives against human immuniodeficiency virus depends on their interaction with CXCR4 chemokine receptor. Mol Pharmacol 1999;55:67-73.  Back to cited text no. 100    
101.Donzella GA, Schols D, Lin SW, Este JA, Nagashima KA, Maddon PJ, et al . AMD3100, a small molecule inhibitor of HIV-1 entry via the CXCR4 co-receptor. Nat Med 1998;4:72-7.  Back to cited text no. 101    
102.Schols D, Este JA, Henson G, De Clercq E. Bicyclams, a class of potent anti HIV agents, are targeted at the HIV co-receptor fusin/CXCR4. Antiviral Res 1997;35:147-56.   Back to cited text no. 102 Clercq E. The bicyclam AMD3100 story. Nat Rev Drug Discov 2003;2:581-7.  Back to cited text no. 103    
104.Ichiyama K, Yokoyama-Kumakura S, Tanaka Y, Tanaka R, Hirose K, Bannai K, et al . A duodenally absorbable CXC chemokine receptor 4 antagonist, KRH 1636, exhibits a potent and selective anti-HIV-1 activity. Proc Natl Acad Sci USA 2003;100:4185-90.  Back to cited text no. 104    
105.Chen JD, Bai X, Yang AG, Cong Y, Chen SY. Inactivation of HIV-1 chemokine co-receptor CXCR4 by a novel intrakine strategy. Nat Med 1997;3:1110-6.  Back to cited text no. 105    
106.Martinez MA, Clotet B, Este JA. RNA interference of HIV replication. Trends Immunol 2002;23:559-61.  Back to cited text no. 106    
107.Martinez MA, Gutierrez A, Armand-Ugon M, Blanco J, Parera M, Gomez J, et al . Suppression of chemokine receptor expression by RNA interference allows for inhibition of HIV-1 replication. AIDS 2002;16:2385-90.  Back to cited text no. 107    
108.Goila R, Banerjea AC. Sequence specific cleavage of HIV-1 co-receptor CCR5 gene by a hammer-head ribozyme and a DNA-enzyme: Inhibition of the co-receptor functions by DNA-enzymes. FEBS Lett 1998;436:233-8.  Back to cited text no. 108    
109.Lederman MM, Veazey RS, Offord R, Mosier DE, Dufour J, Mefford M, et al . Prevention of vaginal SHIV transmission in Rhesus macaques through inhibition of CCR5. Science 2004;306:485-7.   Back to cited text no. 109    
110.Kawamura T, Bruse SE, Abraha A, Sugaya M, Hartley O, Offord RE, et al . PSC-RANTES blocks R5 human immunodeficiency virus infection of Langerhans cells isolated from individuals with a variety of CCR5 diplotypes. J Virol 2004;78:7602-9.  Back to cited text no. 110    
111.Veazey RS, Klasse PJ, Ketas TJ, Reeves JD, Piatak M Jr, Kunstman K, et al . Use of a small molecule CCR5 inhibitor in macaques to treat Simian Immunodeficiency Virus infection or prevent simian-human immunodeficiency virus infection. J Exp Med 2003;198:1551-62.  Back to cited text no. 111    
112.Veazey RS, Klasse PJ, Schader SM, Hu Q, Ketas TJ, Lu M, et al . Protection of macaques from vaginal SHIV challenge by vaginally delivered inhibitors of virus-cell fusion. Nature 2005;438:99-102.  Back to cited text no. 112    
113.Zou YR, Kottmann AH, Kuroda M, Taniuchi I, Littman DR. Function of the chemokine receptor CXCR4 in hematopoiesis and in cerebellar development. Nature 1998;393:595-9.   Back to cited text no. 113    
114.Tachibana K, Hirota S, Iizasa H, Yoshida H, Kawabata K, Kataoka Y, et al . The chemokine receptor CXCR4 is essential for vascularization of gastrointestinal tract. Nature 1998;393:591-4.   Back to cited text no. 114    
115.Nagasawa T, Hirota S, Tachibana K, Takakura M, Nishikawa S, Kitamura Y, et al . Defects of B cell lymphopoiesis and bone marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 1996;382:635-8.   Back to cited text no. 115    
116.Karlsson I, Antonsson L, Shi Y, Oberg M, Karlsson A, Albert J, et al . Coevolution of RANTES sensitivity and mode of CCR5 receptor use by human immunodeficiency virus type 1 of the R5 phenotype. J Virol 2004;78:11807-15.   Back to cited text no. 116    
117.Trkola A, Kuhmann SE, Strizki JM, Maxwell E, Ketas T, Morgan T, et al . HIV-1 escape from a small molecule, CCR5-specific entry inhibitor does not involve CXCR4 use. Proc Natl Acad Sci USA 2002;99:395-400.  Back to cited text no. 117    
118.Reeves JD, Gallo SA, Ahmad N, Miamidian JL, Harvey PE, Sharron M, et al . Sensitivity of HIV-1 to entry inhibitors correlates with envelope/co-receptor affinity, receptor density and fusion kinetics. Proc Natl Acad Sci USA 2002;99:16249-54.  Back to cited text no. 118    


[Table - 1]

This article has been cited by
1 Peptide drugs to target G protein-coupled receptors
Bellmann-Sickert, K., Beck-Sickinger, A.G.
Trends in Pharmacological Sciences. 2010; 31(9): 434-441
2 Potent and broad neutralizing activity of a single chain antibody fragment against cell-free and cell-associated HIV-1
Zhang, M.-Y., Borges, A.R., Ptak, R.G., Wang, Y., Dimitrov, A.S., Alam, S.M., Wieczorek, L., (...), Dimitrov, D.S.
mAbs. 2010; 2(3): 266-274
3 Different plasma levels of interleukins and chemokines: comparison between children and adults with AIDS in China
Chang-zhong, JIN and Yan, Z. and Fu-jie, Z. and Hang-ping, YAO and Ling-jiao, WU and Hong-xin, Z. and Hong-shan, WEI and Nan-ping, WU
Chinese Medical Journal. 2009; 122(5): 530-535
4 Back-burning to cure HIV: Temporary depletion of all CD4+ cells and elimination of the extracellular reservoir with HIV Immunotoxin Therapy
Zanin, M.K.B. and Duvall, M.R.
Medical Hypotheses. 2009; 72(5): 592-595
5 The chemokine system and CCR5 antagonists: potential in HIV treatment and other novel therapies.
Dhami, H. and Fritz, CE and Gankin, B. and Pak, SH and Yi, W. and Seya, M.J. and Raffa, RB and Nagar, S.
Journal of Clinical Pharmacy \& Therapeutics. 2009; 34(2): 147-160
6 Future targets for immune therapy in colitis?
Kristensen, NN and Claesson, MH
Endocrine, metabolic \& immune disorders drug targets. 2008; 8(4): 295-300
7 Molecular cloning and functional analysis of zebrafish (Danio rerio) chemokine genes
Chen, L.-C., Chen, J.-Y., Hour, A.-L., Shiau, C.-Y., Hui, C.-F., Wu, J.-L.
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2008; 151(4): 400-409
8 Gene expression analysis of bromine-induced burns in porcine skin
Price, J.A., Rogers, J.V., McDougal, J.N., Shaw, M.Q., Reid, F.M., Kiser, R.C., Graham, J.S.
Toxicology Letters. 2008; 182(1-3): 69-78
9 Mediators involved in HIV-related pulmonary arterial hypertension.
Humbert, M.
AIDS. 2008; 22((SUPPL 3)): S41-S47
10 Induction of a soluble anti-HIV-1 factor (s) with IFN-γ, IL-10, and β-chemokine modulating activity by an influenza-bacterial polyantigenic mixture
Rodríguez, J.W., Pagan, N.O., Ocasio, M.C., Ríos, Z., Cubano, L.A., Boukli, N.M., Otero, M., (...), Rios-Olivares, E.
American Journal of Infectious Diseases. 2007; 3(4): 266-274
11 Successful HAART is associated with high B-chemokine levels in chronic HIV type 1-infected patients
Brito, A., Almeida, A., Gonsalez, C.R., Mendonça, M., Ferreira, F., Fernandes, S.S., Duarte, A.J.S., Casseb, J.
AIDS Research and Human Retroviruses. 2007; 23(7): 906-912
12 New Therapies for HIV Infection
Spooner, L.M., Olin, J.L.
US Pharm. 2007; 32(10): HS 14-HS 23


Print this article  Email this article
Previous article Next article
Online since 12th February '04
© 2004 - Journal of Postgraduate Medicine
Official Publication of the Staff Society of the Seth GS Medical College and KEM Hospital, Mumbai, India
Published by Wolters Kluwer - Medknow