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Gel purified lipl32: A prospective antigen for detection of leptospirosis
P Tahiliani1, M Mohan Kumar1, D Chandu1, A Kumar1, C Nagaraj2, D Nandi1
1 Dept. of Biochemistry, Indian Institute of Science, Bangalore-560012, India
2 Regional Office for Health and Family Welfare,Government of India, Kormangala,Bangalore-560034, India
Correspondence Address:
D Nandi
Dept. of Biochemistry, Indian Institute of Science, Bangalore-560012
India
Background: Leptospirosis, a zoonosis, is a re-emerging disease, affecting populations across the globe. However, the current methods of diagnosis are time- consuming, cumbersome, imprecise or expensive. Aim: To develop an assay for differential and early diagnosis of Leptospirosis. Methods and Material: IgG based ELISA for evaluation of three antigens, namely, a gel-purified recombinant protein (rLipL32), secreted proteins and whole organism sonicates of Leptospira spp. The antigens were evaluated using, rabbit polyclonal antiserum and human sera samples. Results: Studies with a rabbit polyclonal antiserum indicated the utility of these antigens in differentiating Leptospira from other common pathogenic organisms. Evaluation of these antigens with fifteen representative human serum samples indicated gel-purified rLipL32 to be a potentially useful antigen for detection of leptospirosis. The results obtained with IgG ELISA were correlated with the results of microscopic agglutination test (MAT). Conclusion: Gel-purified rLipL32 is a valuable antigen for early and accurate diagnosis of leptospirosis. Further evaluation of this assay in field conditions and larger sera samples will indicate its suitability in case of an epidemic.
How to cite this article:
Tahiliani P, Kumar M M, Chandu D, Kumar A, Nagaraj C, Nandi D. Gel purified lipl32: A prospective antigen for detection of leptospirosis.J Postgrad Med 2005;51:164-168
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How to cite this URL:
Tahiliani P, Kumar M M, Chandu D, Kumar A, Nagaraj C, Nandi D. Gel purified lipl32: A prospective antigen for detection of leptospirosis. J Postgrad Med [serial online] 2005 [cited 2023 Oct 3 ];51:164-168
Available from: https://www.jpgmonline.com/article.asp?issn=0022-3859;year=2005;volume=51;issue=3;spage=164;epage=168;aulast=Tahiliani;type=0 |
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