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Figure 1: Agarose gel electrophoresis photographs of cases tested for TKD mutations with ASO-PCR. Amplicon lengths- T315I = 158 bp, M244V = 227 bp, E255 K = 194 bp, G250E = 255 bp, Wild kinase domain = 374 bp. (G250E ASO-PCR was biplexed with wild type kinase domain). NC: Negative control (Non –CML sample), NTC: Negative for template control (PCR mastermix without any template added), PC: Positive control, NEG: Negative for mutation, POS: Positive for mutation tested

Figure 1:  Agarose gel electrophoresis photographs of cases tested for TKD mutations with ASO-PCR. Amplicon lengths- T315I = 158 bp, M244V = 227 bp, E255 K = 194 bp, G250E = 255 bp, Wild kinase domain = 374 bp. (G250E ASO-PCR was biplexed with wild type kinase domain). NC: Negative control (Non –CML sample), NTC: Negative for template control (PCR mastermix without any template added), PC: Positive control, NEG: Negative for mutation, POS: Positive for mutation tested